Goat IGFBP1 Polyclonal Antibody | anti-IGFBP1 antibody

Goat Polyclonal to Human IGFBP1

Cat. Num. AAA248851

• Gene Names: IGFBP1; AFBP; IBP1; PP12; IGF-BP25; hIGFBP-1
• Reactivity: Chimpanzee, Gorilla, Human, Orangutan
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: IGFBP1; Polyclonal Antibody; Goat Polyclonal to Human IGFBP1; Anti-IGFBP1 Antibody (aa156-170) IHC-plus; AFBP; Amniotic fluid binding protein; Binding protein-26; Binding protein-25; Binding protein-28; IGF-binding protein 1; IGF-BP25; IBP1; HIGFBP-1; PP12; Placental protein 12; IBP-1; IGFBP-1; Human IGFBP1; anti-IGFBP1 antibody

• Host: Goat
• Reactivity: Chimpanzee, Gorilla, Human, Orangutan
• Clonality: Polyclonal
• Specificity: Human IGFBP1 / IGFBP-1
• Purity/Purification: Immunoaffinity Purified
• Form/Format: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 259

• Applicable Applications for anti-IGFBP1 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:64000), IHC-P (10 ug/ml), WB (1 - 3 ug/ml); Usage: Peptide ELISA: antibody detection limit dilution 1:64000. Western blot: Approx 30kD band observed in Human Placenta lysates (calculated MW of 27.9kD according to NP_000587.1). Recommended concentration: 1-3 ug/ml. An additional band of 55kD was consi...
• Target Species: Human
• Immunogen Description: Synthetic peptide C-STYDGSKALHVTNIK from an internal region of human IGFBP1 / IGFBP-1 (NP_000587.1). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan (100%); Gibbon (93%).
• Immunogen Type: Synthetic peptide
• Immunogen: IGFBP1 antibody was raised against synthetic peptide C-STYDGSKALHVTNIK from an internal region of human IGFBP1 / IGFBP-1 (NP_000587.1). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan (100%); Gibbon (93%).
• Antigen Modification: aa156-170
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-IGFBP1 antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Western Blot (WB)

(IGFBP1 antibody (2 ug/ml) staining of Human Placenta lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

• NCBI GI #: 4504615
• NCBI GeneID: 3484
• NCBI Accession #: NP_000587.1
• NCBI GenBank Nucleotide #: NM_000596.2
• UniProt Accession #: P08833; Q8IYP5; A4D2F4; D3DVL9
• Molecular Weight: 27,904 Da
• NCBI Official Full Name: insulin-like growth factor-binding protein 1
• NCBI Official Synonym Full Names: insulin-like growth factor binding protein 1
• NCBI Official Symbol: IGFBP1
• NCBI Official Synonym Symbols: AFBP; IBP1; PP12; IGF-BP25; hIGFBP-1
• NCBI Protein Information: insulin-like growth factor-binding protein 1; IBP-1; IGFBP-1; binding protein-25; binding protein-26; binding protein-28; placental protein 12; IGF-binding protein 1; amniotic fluid binding protein; growth hormone independent-binding protein; alpha-pregnancy-associated endometrial globulin
• UniProt Protein Name: Insulin-like growth factor-binding protein 1
• Protein Family: Insulin-like growth factor-binding protein
• UniProt Gene Name: IGFBP1
• UniProt Synonym Gene Names: IBP1; IBP-1; IGF-binding protein 1; IGFBP-1; PP12
• UniProt Entry Name: IBP1_HUMAN

NCBI Description

This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein binds both insulin-like growth factors (IGFs) I and II and circulates in the plasma. Binding of this protein prolongs the half-life of the IGFs and alters their interaction with cell surface receptors. [provided by RefSeq, Jul 2008]

Uniprot Description

IGFBP1: IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Promotes cell migration.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 7p12.3

Cellular Component: extracellular space; extracellular region

Molecular Function: insulin-like growth factor binding; insulin-like growth factor I binding; insulin-like growth factor II binding

Biological Process: regulation of insulin-like growth factor receptor signaling pathway; unfolded protein response, activation of signaling protein activity; cellular protein metabolic process; unfolded protein response; tissue regeneration; insulin receptor signaling pathway; signal transduction; positive regulation of cell growth