Human Peptidyl Arginine Deiminase Type III (PADI3) ELISA Kit | PADI3 elisa kit

Human Peptidyl Arginine Deiminase Type III (PADI3) ELISA Kit

Cat. Num. AAA2024827

• Gene Names: PADI3; PAD3; PDI3
• Reactivity: Human
• Synonyms: Peptidyl Arginine Deiminase Type III (PADI3); Human Peptidyl Arginine Deiminase Type III (PADI3) ELISA Kit; PAD3; PDI3; Peptidylarginine deiminase III; PADI3 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Peptidyl Arginine Deiminase Type III (PADI3).; No significant cross-reactivity or interference between Peptidyl Arginine Deiminase Type III (PADI3) and analogues was observed.
• Sequence Length: 664

• Assay Type: Double-antibody Sandwich
• Samples: Saliva, Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids
• Detection Range: 0.78 -50ng/mL
• Sensitivity: < 0.31ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Peptidyl Arginine Deiminase Type III (PADI3) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Peptidyl Arginine Deiminase Type III (PADI3) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PADI3 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Peptidyl Arginine Deiminase Type III (PADI3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Peptidyl Arginine Deiminase Type III (PADI3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Peptidyl Arginine Deiminase Type III (PADI3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Peptidyl Arginine Deiminase Type III (PADI3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for PADI3 elisa kit

Enzyme & Kinase

NCBI and Uniprot Product Information

• NCBI GI #: 122939161
• NCBI GeneID: 51702
• NCBI Accession #: NP_057317.2
• NCBI GenBank Nucleotide #: NM_016233.2
• Molecular Weight: 74,743 Da
• NCBI Official Full Name: protein-arginine deiminase type-3
• NCBI Official Synonym Full Names: peptidyl arginine deiminase, type III
• NCBI Official Symbol: PADI3
• NCBI Official Synonym Symbols: PAD3; PDI3
• NCBI Protein Information: protein-arginine deiminase type-3
• UniProt Protein Name: Protein-arginine deiminase type-3
• Protein Family: Protein-arginine deiminase
• UniProt Gene Name: PADI3
• UniProt Synonym Gene Names: PAD3; PDI3
• UniProt Entry Name: PADI3_HUMAN

NCBI Description

This gene encodes a member of the peptidyl arginine deiminase family of enzymes, which catalyze the post-translational deimination of proteins by converting arginine residues into citrullines in the presence of calcium ions. The family members have distinct substrate specificities and tissue-specific expression patterns. The type III enzyme modulates hair structural proteins, such as filaggrin in the hair follicle and trichohyalin in the inner root sheath, during hair follicle formation. Together with the type I enzyme, this enzyme may also play a role in terminal differentiation of the epidermis. This gene exists in a cluster with four other paralogous genes. [provided by RefSeq, Jul 2008]

Uniprot Description

PADI3: Catalyzes the deimination of arginine residues of proteins. Belongs to the protein arginine deiminase family.

Protein type: EC 3.5.3.15; Hydrolase

Chromosomal Location of Human Ortholog: 1p36.13

Cellular Component: cytoplasm

Molecular Function: calcium ion binding; protein-arginine deiminase activity

Biological Process: peptidyl-citrulline biosynthetic process from peptidyl-arginine