Enterovirus 71 VP0 Recombinant Protein | VP0 recombinant protein

Human Enterovirus 71 VP0 Protein (His & GST Tag)

Cat. Num. AAA8118705

• Purity: >85 % as determined by SDS-PAGE
• Synonyms: Enterovirus 71 VP0; Human Enterovirus 71 VP0 Protein (His & GST Tag); VP0 Protein; EV71; VP4-VP2 Protein; VP0 recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: >85 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 50mM Tris, 100mM NaCl, 2mM GSH, 0.5mM PMSF, pH8.0 Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Sequence Positions: 560aa
• Sequence: Met1-Gln323

• Species: Human Enterovirus 71
• Tag: N-GST & His
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Met
• Protein Construction: |A DNA sequence encoding the amino acids (Met 1-Gln 323) of human enterovirus 71 genome polyprotein (Q66478-1), corresponding to the protein VP0 precusor, a component of immature procapsids, was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; Shipping: They are shipped out with blue ice pack

Testing Data

Related Product Information for VP0 recombinant protein

Human enterovirus 71 genome polyprotein is a member of the picornaviruses polyprotein family. It contains two peptidase C3 domains, one RdRp catalytic domain, one SF3 helicase domain. Genome polyprotein is cleaved into the following 12 chains: Protein VP (VP4-VP2), Protein VP4 (P1A), Protein VP2 (P1B), Protein VP3 (P1C), Protein VP1 (P1D), Picornain 2A (P2A), Protein 2B (P2B), Protein 2C (P2C), Protein 3A (P3A), Protein 3B (P3B), Picornain 3C (Protease 3C) and RNA-directed RNA polymerase 3D-POL (P3D-POL). VP precursor is a component of immature procapsids. Capsid proteins VP1, VP2, VP3 and VP4 form a closed capsid enclosing the viral positive strand RNA genome. VP4 lies on the inner surface of the protein shell formed by VP1, VP2 and VP3. All the three latter proteins contain a beta-sheet structure called beta-barrel jelly roll. Together they form an icosahedral capsid composed of 6 copies of each VP1, VP2, and VP3, with a diameter of approximately 3 Angstroms. VP1 is situated at the 12 fivefold axes, whereas VP2 and VP3 are located at the quasi-sixfold axes.

References

Brown B.A., et al., 1995, Virus Res. 39:195-206. Tang W.-F. et al., 2007, J. Biol. Chem. 282:5888-5898. Huang, S.C. et al., 2008, Virus Res. 131 (2):250-9.

NCBI and Uniprot Product Information

• NCBI GI #: 2499561
• UniProt Accession #: Q66478
• Molecular Weight: 242,852 Da
• NCBI Official Full Name: Genome polyprotein
• UniProt Protein Name: Genome polyprotein
• UniProt Gene Name: P2A
• UniProt Synonym Gene Names: P2B; P2C; P3A; VPg; P3B; P3C; RdRp; 3Dpol; 3D

Uniprot Description

Capsid protein VP1: Forms an icosahedral capsid of pseudo T=3 symmetry with capsid proteins VP2 and VP3. The capsid is 300 Angstroms in diameter, composed of 60 copies of each capsid protein and enclosing the viral positive strand RNA genome. Capsid protein VP1 mainly forms the vertices of the capsid. Capsid protein VP1 interacts with host cell receptor to provide virion attachment to target host cells. This attachment induces virion internalization. Tyrosine kinases are probably involved in the entry process. After binding to its receptor, the capsid undergoes conformational changes. Capsid protein VP1 N-terminus (that contains an amphipathic alpha-helix) and capsid protein VP4 are externalized. Together, they shape a pore in the host membrane through which viral genome is translocated to host cell cytoplasm. After genome has been released, the channel shrinks ().