FA10 Antibody | anti-FA10 antibody

FA10 (activated heavy chain, Cleaved-Ile235) Antibody

Cat. Num. AAA855160

• Gene Names: F10; FX; FXA
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, ELISA
• Purity: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: FA10; FA10 (activated heavy chain; Cleaved-Ile235) Antibody; coagulation factor X; EC 3.4.21.6; FX; FXA; Stuart factor; Stuart-Prower factor; anti-FA10 antibody

• Reactivity: Human, Mouse, Rat
• Specificity: FA10 (activated heavy chain, Cleaved-Ile235) antibody detects endogenous levels of fragment of activated FA10 resulting from cleavage adjacent to Ile235.
• Purity/Purification: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 488

• Applicable Applications for anti-FA10 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:500~1:1000; ELISA: 1:20000
• Immunogen: The antiserum was produced against synthesized peptide derived from human FA10.
• Preparation and Storage: Store at-20 degree C for 1 year.

Western Blot (WB)

(Western blot analysis of extracts from rat liver cells, using FA10 (activated heavy chain, Cleaved-Ile235) antibody.)

References

Messier T.L., Gene 99:291-294(1991). Leytus S.P., Biochemistry 25:5098-5102(1986). Fung M.R., Proc. Natl. Acad. Sci. U.S.A. 82:3591-3595(1985).

NCBI and Uniprot Product Information

• NCBI GI #: 4503625
• NCBI GeneID: 2159
• NCBI Accession #: NP_000495.1
• NCBI GenBank Nucleotide #: NM_000504.3
• UniProt Accession #: P00742; Q14340
• Molecular Weight: 54,732 Da
• NCBI Official Full Name: coagulation factor X isoform 1 preproprotein
• NCBI Official Synonym Full Names: coagulation factor X
• NCBI Official Symbol: F10
• NCBI Official Synonym Symbols: FX; FXA
• NCBI Protein Information: coagulation factor X
• UniProt Protein Name: Coagulation factor X
• UniProt Gene Name: F10

NCBI Description

This gene encodes the vitamin K-dependent coagulation factor X of the blood coagulation cascade. This factor undergoes multiple processing steps before its preproprotein is converted to a mature two-chain form by the excision of the tripeptide RKR. Two chains of the factor are held together by 1 or more disulfide bonds; the light chain contains 2 EGF-like domains, while the heavy chain contains the catalytic domain which is structurally homologous to those of the other hemostatic serine proteases. The mature factor is activated by the cleavage of the activation peptide by factor IXa (in the intrisic pathway), or by factor VIIa (in the extrinsic pathway). The activated factor then converts prothrombin to thrombin in the presence of factor Va, Ca+2, and phospholipid during blood clotting. Mutations of this gene result in factor X deficiency, a hemorrhagic condition of variable severity. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar proteolytic processing to generate mature polypeptides. [provided by RefSeq, Aug 2015]

Uniprot Description

Factor Xa is a vitamin K-dependent glycoprotein that converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting.