OBCAM / OPCML recombinant protein

Recombinant Human OBCAM / OPCML Protein (His tag)

Cat. Num. AAA2545170

• Gene Names: OPCML; OPCM; OBCAM; IGLON1
• Purity: > 95 % as determined by SDS-PAGE
• Synonyms: OBCAM / OPCML; Recombinant Human OBCAM / OPCML Protein (His tag); OBCAM; OPCM; OPCML; OBCAM / OPCML recombinant protein

• Host: Human Cells
• Purity/Purification: > 95 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 345

• Application Notes: The mature recombinant human OPCML consists of 306 amino acids and has a calculated molecular mass of 34 kDa. As a result of glycosylation, rhOPCML migrates as an approximately 55-60 kDa protein in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Gly 28
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

Related Product Information for OBCAM / OPCML recombinant protein

Background: Opioid-binding Cell Adhesion Molecule (OBCAM), also known as OPCML, is a GPI-anchored cell adhesion molecule in the plasma membrane. This neuron-specific protein, consists of three immunoglobulin (Ig)-like domains anchored to the membrane through a glycosylphosphatidylinositol (GPI)-tail. OPCML also belongs to the member of the IgLON family, a subgroup of the immunoglobulin superfamily, consisting of three members, LAMP, OBCAM, and Neurotrimin. These molecules interact homophilically and heterophilically within the family, and OBCAM acts only as heterodimers with LAMP or Neurotrimin and possibly inhibits neurite outgrowth from cerebellar granule cells. OBCAM has been presumed to play a role as a cell adhesion/recognition molecule. Furthermore, the OPCML protein defects may play an important role in the carcinogenesis of cervical or ovarian cancers, and this gene is regarded as a candidate TSG (tumor suppressor gene).

Description: A DNA sequence encoding the human OPCML precursor (NP_002536.1) (Met 1-Asn 322) was expressed with a C-terminal polyhistidine tag.

NCBI and Uniprot Product Information

• NCBI GI #: 4505505
• NCBI GeneID: 4978
• NCBI Accession #: NP_002536.1
• NCBI GenBank Nucleotide #: NM_002545.4
• UniProt Accession #: Q14982; Q17RN7; Q7Z3W6; B2CZX2; B7ZLQ1
• Molecular Weight: 38,849 Da
• NCBI Official Full Name: opioid-binding protein/cell adhesion molecule isoform a preproprotein
• NCBI Official Synonym Full Names: opioid binding protein/cell adhesion molecule like
• NCBI Official Symbol: OPCML
• NCBI Official Synonym Symbols: OPCM; OBCAM; IGLON1
• NCBI Protein Information: opioid-binding protein/cell adhesion molecule
• UniProt Protein Name: Opioid-binding protein/cell adhesion molecule
• UniProt Gene Name: OPCML
• UniProt Synonym Gene Names: IGLON1; OBCAM; OBCAM; OPCML; Opioid-binding cell adhesion molecule

NCBI Description

This gene encodes a member of the IgLON subfamily in the immunoglobulin protein superfamily of proteins. The encoded preprotein is proteolytically processed to generate the mature protein. This protein is localized in the plasma membrane and may have an accessory role in opioid receptor function. This gene has an ortholog in rat and bovine. The opioid binding-cell adhesion molecule encoded by the rat gene binds opioid alkaloids in the presence of acidic lipids, exhibits selectivity for mu ligands and acts as a GPI-anchored protein. Since the encoded protein is highly conserved in species during evolution, it may have a fundamental role in mammalian systems. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Jan 2016]

Uniprot Description

OPCML: Binds opioids in the presence of acidic lipids; probably involved in cell contact. Defects in OPCML are a cause of susceptibility to ovarian cancer (OC). Ovarian cancer common malignancy originating from ovarian tissue. Although many histologic types of ovarian neoplasms have been described, epithelial ovarian carcinoma is the most common form. Ovarian cancers are often asymptomatic and the recognized signs and symptoms, even of late- stage disease, are vague. Consequently, most patients are diagnosed with advanced disease. Belongs to the immunoglobulin superfamily. IgLON family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, GPI anchor; Receptor, misc.

Chromosomal Location of Human Ortholog: 11q25

Biological Process: neuron recognition

Disease: Ovarian Cancer