Testing Data
Carboxypeptidase B1 ELISA Pair Set | CPB1 elisa pair set
Carboxypeptidase B1 Matched ELISA Antibody Pair Set, Mouse
Synonyms
Carboxypeptidase B1; Carboxypeptidase B1 Matched ELISA Antibody Pair Set; Mouse; Mouse Carboxypeptidase B1/CPB1 ELISA Pair Set; carboxypeptidase B1 (tissue); 0910001A18Rik Matched ELISA Antibody Pair Set; 1810063F02Rik Matched ELISA Antibody Pair Set; 2210008M23Rik Matched ELISA Antibody Pair Set; AI504870 Matched ELISA Antibody Pair Set; CPB1 elisa pair set
Specificity
Quantitative determination of Mouse Carboxypeptidase B1
Assay Type
Solid Phase Sandwich ELISA
Detection Range
12.5-800pg/mL
Sensitivity
12.5pg/ml
Materials Provided
1. Capture Antibody 1.0 mg/mL of rabbit anti-mouse CPB1 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2ug/mL in CBS before coating.
2. Detection Antibody 0.5 mg/mL rabbit anti-mouse CPB1 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % glycerol, pH 7.4). Dilute to working concentration of 0.5ug/mL in detection antibody dilution buffer before use.
3. Standard Each vial contains 39 ng of recombinant mouse CPB1. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 0.8ng/mL is recommended.
2. Detection Antibody 0.5 mg/mL rabbit anti-mouse CPB1 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % glycerol, pH 7.4). Dilute to working concentration of 0.5ug/mL in detection antibody dilution buffer before use.
3. Standard Each vial contains 39 ng of recombinant mouse CPB1. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 0.8ng/mL is recommended.
Preparation and Storage
Capture Antibody: Aliquot and store at -20 degree C to -80 degree C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20 degree C to -80 degree C and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.
Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20 degree C to -80 degree C and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.
Testing Data
Testing Data
Related Product Information for CPB1 elisa pair set
Carboxypeptidase B1, also well known as pancreatic procarboxypeptidase B (PCPB), is a highly pancreas -specific protein (PASP), and has been identified previously as a serum marker for acute pancreatitis and pancreatic graft rejection. As the prototype for those human exopeptidases that cleave off basic C-terminal residues, CPB1 specifically cleaves the C-terminal Arg and Lys residues with a preference for Arg. The B1 and B2 forms of procarboxypeptidase B differ from each other mainly in isoelectric point.The deduced amino acid sequence of PCPB predicts a 416-amino acid preproenzyme consisting of a 15-aa signal peptide, a 95-aa activation peptide and a 37-aa mature chain. The secreted PCPB zymogen is converted to enzymatically active CPB1 by limited proteolysis by trypsin.
This Carboxypeptidase B1 Matched ELISA Antibody Pair Set, Mouse is a solid phase sandwich ELISA for quantitative determination of Mouse Carboxypeptidase B1. It contains Mouse Carboxypeptidase B1 capture antibody, Mouse Carboxypeptidase B1 detector antibody (HRP) and a highly purified HEK293-expressed recombinant Mouse Carboxypeptidase B1 protein.
This Carboxypeptidase B1 Matched ELISA Antibody Pair Set, Mouse is a solid phase sandwich ELISA for quantitative determination of Mouse Carboxypeptidase B1. It contains Mouse Carboxypeptidase B1 capture antibody, Mouse Carboxypeptidase B1 detector antibody (HRP) and a highly purified HEK293-expressed recombinant Mouse Carboxypeptidase B1 protein.
References
Yamamoto, K.K. et al., 1992, J. Biol. Chem. 267: 2575-2581. Pezzilli, R. et al., 1994, Digestion. 55: 73-77. Barbosa Pereira, P.J. et al., 2002, J. Mol. Biol. 321: 537-547.
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Product Notes
The CPB1 (Catalog #AAA8118494) is an ELISA Pair Set and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Carboxypeptidase B1, ELISA Pair Set" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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