Rabbit PRRX2 Polyclonal Antibody | anti-PRRX2 antibody

PRRX2 Polyclonal Antibody

Cat. Num. AAA3006454

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: PRRX2; Polyclonal Antibody; PRRX2 Polyclonal Antibody; Paired mesoderm homeobox protein 2; Paired-related homeobox protein 2; PRX-2; PMX2; PRX2; anti-PRRX2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: PRRX2 polyclonal antibody detects endogenous levels of PRRX2 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 253

• Applicable Applications for anti-PRRX2 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: A synthetic peptide corresponding to residues in Human PRRX2.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of PRRX2 polyclonal antibody at 1:500 dilutionLane1:HEK293T whole cell lysateLane2:sp2/0 whole cell lysateLane3:PC12 whole cell lysate)

Related Product Information for anti-PRRX2 antibody

The paired-class homeobox genes PRX1 and PRX2 are necessary for craniofacial and limb development and are expressed in similar patterns in the cranial mesenchyme, limb buds, axial mesoderm and branchial arches. These proteins exhibit different patterns of expression, however, in heart and brain tissue. Specifically, PRX1 is expressed in the endocardial cusions, semilunar and atrioventricular valves, whereas PRX2 is initially expressed in a diffuse myocardial pattern and is later expressed in the ventricular septum. Furthermore, PRX2 is never expressed in the brain, whereas PRX1 is expressed in the ventral hypothalamus and in the telencephalon. Murine mutants lacking PRX1 function demonstrate skeletal defects in the skull, limbs, and vertebral column. Mice lacking functional PRX2 alone do not demonstrate skeletal abnormalities, however, PRX1/PRX2 double mutants demonstrate novel abnormalities that are not visualized with the PRX1-deficient mice. Transcripts of neither PRX1 nor PRX2 are detected in normal adult rat pulmonary arteries, however vasular disease induces PRX gene expression wherein they co-localize to sites of Tenascin-C expression.

NCBI and Uniprot Product Information

• NCBI GI #: 7706659
• NCBI Accession #: NP_057391.1
• NCBI GenBank Nucleotide #: NP_057391.1
• UniProt Accession #: Q99811
• Molecular Weight: ~ 27kDa
• NCBI Official Full Name: paired mesoderm homeobox protein 2
• UniProt Protein Name: Paired mesoderm homeobox protein 2
• Protein Family: Paired mesoderm homeobox protein
• UniProt Gene Name: PRRX2
• UniProt Synonym Gene Names: PMX2; PRX2; PRX-2
• UniProt Entry Name: PRRX2_HUMAN

Uniprot Description

Function: May play a role in the scarless healing of cutaneous wounds during the first two trimesters of development. Ref.5

Subcellular location: Nucleus

By similarity.

Tissue specificity: In fetal skin, highest expression found in cells of mesodermal origin within the dermal papilla of the developing hair shaft. Not detected in epidermis or dermis. In adult skin, weakly expressed within the basal layers of the epidermis. Not expressed in dermis. Ref.5

Developmental stage: Higher expression in fetus than in adult. Ref.5

Sequence similarities: Belongs to the paired homeobox family.Contains 1 homeobox DNA-binding domain.