Typical Testing Data/Standard Curve (for reference only)
Mimecan ELISA Kit | Ogn elisa kit
Mouse Mimecan PicoKine ELISA Kit
Gene Names
Ogn; OG; OIF; SLRR3A; mimecan; mimican; 3110079A16Rik
Reactivity
MouseThere is no detectable cross-reactivity with other relevant proteins.
Applications
ELISA
Synonyms
Mimecan; Mouse Mimecan PicoKine ELISA Kit; Osteoglycin; Ogn; Og; Ogn elisa kit
Reactivity
Mouse
There is no detectable cross-reactivity with other relevant proteins.
There is no detectable cross-reactivity with other relevant proteins.
Specificity
Natural and recombinant Mouse Mimecan
Sequence Length
298
Applicable Applications for Ogn elisa kit
ELISA (EIA)
Assay Type
Quantitative Sandwich
Detection Range
62.5pg/ml-4000pg/ml
Sensitivity
<12pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Ogn elisa kit
Principle of the Assay: Mouse Mimecan ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A a monoclonal antibody from rat specific for Mimecan has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: A20-F298) and test samples are added to the wells, a biotinylated detection a biotinylated polyclonal antibody from goat specific for Mimecan is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse Mimecan amount of sample captured in plate.
Background: Osteoglycin (also called mimecan) is a protein that in humans is encoded by the OGN gene. This gene encodes a protein which induces ectopic bone formation in conjunction with transforming growth factor beta. This protein is a small proteoglycan which contains tandem leucine-rich repeats (LRR). The level of expression of this gene has been correlated with enlarged hearts and more specifically left ventricular hypertrophy. Mimecan belongs to a family of small leucine-rich proteoglycans (SLRPs). The mimecan gene was expressed at a moderate level in the mouse pituitary gland by Northern blot analysis. Expression of mimecan mRNA and protein is also observed in the human anterior pituitary gland. The OGN gene was mapped to chromosome 9q22.
Background: Osteoglycin (also called mimecan) is a protein that in humans is encoded by the OGN gene. This gene encodes a protein which induces ectopic bone formation in conjunction with transforming growth factor beta. This protein is a small proteoglycan which contains tandem leucine-rich repeats (LRR). The level of expression of this gene has been correlated with enlarged hearts and more specifically left ventricular hypertrophy. Mimecan belongs to a family of small leucine-rich proteoglycans (SLRPs). The mimecan gene was expressed at a moderate level in the mouse pituitary gland by Northern blot analysis. Expression of mimecan mRNA and protein is also observed in the human anterior pituitary gland. The OGN gene was mapped to chromosome 9q22.
Product Categories/Family for Ogn elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
34,012 Da
NCBI Official Full Name
mimecan
NCBI Official Synonym Full Names
osteoglycin
NCBI Official Symbol
Ogn
NCBI Official Synonym Symbols
OG; OIF; SLRR3A; mimecan; mimican; 3110079A16Rik
NCBI Protein Information
mimecan
UniProt Protein Name
Mimecan
Protein Family
UniProt Gene Name
Ogn
UniProt Synonym Gene Names
Og
Uniprot Description
Induces bone formation in conjunction with TGF-beta-1 or TGF-beta-2.
Research Articles on Ogn
Similar Products
Product Notes
The Mouse There is no detectable cross-reactivity with other relevant proteins. Ogn ogn (Catalog #AAA1751138) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA1751138 ELISA Kit recognizes Mouse There is no detectable cross-reactivity with other relevant proteins. Ogn. AAA Biotech's Mimecan can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). Researchers should empirically determine the suitability of the Ogn ogn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Mimecan, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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