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Typical Testing Data/Standard Curve (for reference only)

BMP-6 elisa kit

Rat BMP-6 PicoKine ELISA Kit

Gene Names
Bmp6; VGR
Reactivity
Rat
There is no detectable cross-reactivity with other relevant proteins.
Applications
ELISA
Synonyms
BMP-6; Rat BMP-6 PicoKine ELISA Kit; Bone morphogenetic protein 6; VG-1-related protein; VGR-1; Bmp6; Bmp-6; Vgr; BMP-6 elisa kit
Ordering
For Research Use Only!
Reactivity
Rat
There is no detectable cross-reactivity with other relevant proteins.
Specificity
Natural and recombinant Rat BMP-6
Sequence Length
506
Applicable Applications for BMP-6 elisa kit
ELISA (EIA)
Assay Type
Quantitative Sandwich
Detection Range
156pg/ml-10000pg/ml
Sensitivity
<10pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for BMP-6 elisa kit
Principle of the Assay: Rat BMP-6 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A a monoclonal antibody from mouse specific for BMP-6 has been precoated onto 96- well plates. Standards(Expression system for standard: CHO, Immunogen sequence: S368-H506) and test samples are added to the wells, a biotinylated detection a biotinylated polyclonal antibody from goat specific for BMP-6 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat BMP-6 amount of sample captured in plate.

Background: The bone morphogenetic proteins (BMPs) are a family of secreted signaling molecules that can induce ectopic bone growth. Many BMPs are part of the transforming growth factor-beta (TGFB) superfamily. BMPs were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. Based on its expression early in embryogenesis, BMP-6 has a proposed role in early development. In addition, the fact that this BMP is closely related to BMP5 and BMP7 has lead to speculation of possible bone inductive activity.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
56,223 Da
NCBI Official Full Name
bone morphogenetic protein 6
NCBI Official Synonym Full Names
bone morphogenetic protein 6
NCBI Official Symbol
Bmp6
NCBI Official Synonym Symbols
VGR
NCBI Protein Information
bone morphogenetic protein 6
UniProt Protein Name
Bone morphogenetic protein 6
UniProt Gene Name
Bmp6
UniProt Synonym Gene Names
Bmp-6; Vgr; BMP-6; VGR-1

NCBI Description

plays a role in induction of osteogenesis; may be involved in liver growth and differentiation in response to injury [RGD, Feb 2006]

Uniprot Description

Induces cartilage and bone formation.

Research Articles on BMP-6

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Product Notes

The Rat There is no detectable cross-reactivity with other relevant proteins. BMP-6 bmp6 (Catalog #AAA1751131) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA1751131 ELISA Kit recognizes Rat There is no detectable cross-reactivity with other relevant proteins. BMP-6. AAA Biotech's BMP-6 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). Researchers should empirically determine the suitability of the BMP-6 bmp6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BMP-6, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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