Canine Nucleoporin 155kDa ELISA Kit | NUP155 elisa kit

Canine Nucleoporin 155kDa ELISA Kit

Cat. Num. AAA744894

• Gene Names: NUP155; N155; ATFB15
• Reactivity: Canine
• Synonyms: Nucleoporin 155kDa; Canine Nucleoporin 155kDa ELISA Kit; NUP155 elisa kit

• Reactivity: Canine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 0.5-10ng/mL
• Sensitivity: 0.1ng/mL
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NUP155 elisa kit

Intended Uses: This NUP155 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine NUP155. This ELISA kit for research use only!

Principle of the Assay: NUP155 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-NUP155 antibody and an NUP155-HRP conjugate. The assay sample and buffer are incubated together with NUP155-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NUP155 concentration since NUP155 from samples and NUP155-HRP conjugate compete for the anti-NUP155 antibody binding site. Since the number of sites is limited, as more sites are occupied by NUP155 from the sample, fewer sites are left to bind NUP155-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NUP155 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for NUP155 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 24430149
• NCBI GeneID: 9631
• NCBI Accession #: NP_705618.1
• NCBI GenBank Nucleotide #: NM_153485.2
• Molecular Weight: 149,016 Da
• NCBI Official Full Name: nuclear pore complex protein Nup155 isoform 1
• NCBI Official Synonym Full Names: nucleoporin 155kDa
• NCBI Official Symbol: NUP155
• NCBI Official Synonym Symbols: N155; ATFB15
• NCBI Protein Information: nuclear pore complex protein Nup155; nucleoporin 155kD; 155 kDa nucleoporin
• UniProt Protein Name: Nuclear pore complex protein Nup155
• Protein Family: Nuclear pore complex protein
• UniProt Gene Name: NUP155
• UniProt Synonym Gene Names: KIAA0791
• UniProt Entry Name: NU155_HUMAN

NCBI Description

Nucleoporins are proteins that play an important role in the assembly and functioning of the nuclear pore complex (NPC) which regulates the movement of macromolecules across the nuclear envelope (NE). The protein encoded by this gene plays a role in the fusion of NE vesicles and formation of the double membrane NE. The protein may also be involved in cardiac physiology and may be associated with the pathogenesis of atrial fibrillation. Alternative splicing results in multiple transcript variants of this gene. A pseudogene associated with this gene is located on chromosome 6. [provided by RefSeq, May 2013]

Uniprot Description

NUP155: Essential component of nuclear pore complex. Nucleoporins may be involved both in binding and translocating proteins during nucleocytoplasmic transport. Belongs to the non-repetitive/WGA-negative nucleoporin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nucleoporin

Chromosomal Location of Human Ortholog: 5p13.1

Cellular Component: nuclear membrane; membrane; nuclear envelope; nuclear pore

Molecular Function: structural constituent of nuclear pore; transporter activity

Biological Process: viral reproduction; cytokine and chemokine mediated signaling pathway; mitotic nuclear envelope disassembly; pathogenesis; glucose transport; viral infectious cycle; nuclear membrane organization and biogenesis; mRNA export from nucleus; protein import into nucleus; hexose transport; carbohydrate metabolic process; gene expression; viral transcription; mitotic cell cycle; transmembrane transport

Disease: Atrial Fibrillation, Familial, 15