Numb Homolog (NUMB) Recombinant Protein | NUMB recombinant protein

Recombinant Numb Homolog (NUMB)

Cat. Num. AAA2018553

• Gene Names: Numb; Nb
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Numb Homolog (NUMB); Recombinant Numb Homolog (NUMB); NUMB recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- LTAK PANTALAHVA MPVRETNPWA HVPDAANKEI AAIHPGTEWG QSSGAASPGL FQAGHRRTPS EADRWLEEVS KSVRAQQPQV SAAPLQPVLQ PPPPAAIAPP APPFQGHAFL TSQPVPVGVV PPLQPAFVPT QSYPVANGMP YPASNVPVVG ITPSQMVANV FGTAGHPQTT HPHQSPSLAK QQTFPQYETS SATTSPFFKP PAQHLNGSAA FNGVDNGGLA SGNRHAEVPP GTCPVDPFEAQWAALESKSK QRTNPSPTNP FS
• Sequence Length: 653

• Applicable Applications for NUMB recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Leu377~Ser642 (Accession # Q9QZS3) with two N-terminal Tags, His-tag and T7-tag
• Subcellular Location: Membrane; Peripheral membrane protein
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Predicted Isoelectric Point: 8.1
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 209862959
• NCBI GeneID: 18222
• NCBI Accession #: NP_001129547.1
• NCBI GenBank Nucleotide #: NM_001136075.2
• UniProt Accession #: Q9QZS3; P70422; Q8CIB1; Q9DC57; Q9QZR1; Q9QZS4
• Molecular Weight: 31.6kDa
• NCBI Official Full Name: protein numb homolog isoform 1
• NCBI Official Synonym Full Names: numb gene homolog (Drosophila)
• NCBI Official Symbol: Numb
• NCBI Official Synonym Symbols: Nb
• NCBI Protein Information: protein numb homolog
• UniProt Protein Name: Protein numb homolog
• Protein Family: Protein numb
• UniProt Gene Name: Numb
• UniProt Synonym Gene Names: m-Nb; m-Numb
• UniProt Entry Name: NUMB_MOUSE

NCBI Description

This gene encodes a conserved protein that is distributed asymmetrically during cell division in the developing embryo. The encoded protein participates in cell fate decisions by interacting with the Notch receptor. Loss of function of this gene results in severe defects in neural development and loss of viability. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2013]

Uniprot Description

NUMB: Plays a role in the process of neurogenesis. Required throughout embryonic neurogenesis to maintain neural progenitor cells, also called radial glial cells (RGCs), by allowing their daughter cells to choose progenitor over neuronal cell fate. Not required for the proliferation of neural progenitor cells before the onset of neurogenesis. Also involved postnatally in the subventricular zone (SVZ) neurogenesis by regulating SVZ neuroblasts survival and ependymal wall integrity. May also mediate local repair of brain ventricular wall damage. Interacts with CDH1 and TFAP2B. Interacts with EPS15, LNX and NOTCH1. May interact with DUOXA1. Interacts with RALBP1 in a complex also containing EPN1 and TFAP2A during interphase and mitosis. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell development/differentiation

Cellular Component: extrinsic to plasma membrane; focal adhesion; membrane; clathrin-coated vesicle; apical part of cell; basolateral plasma membrane; apical plasma membrane; early endosome; cytoplasmic membrane-bound vesicle; cytoplasm; nucleus

Molecular Function: protein binding; cadherin binding; beta-catenin binding; alpha-catenin binding

Biological Process: positive regulation of polarized epithelial cell differentiation; nervous system development; positive regulation of neurogenesis; axonogenesis; regulation of neuron differentiation; multicellular organismal development; neuroblast proliferation; forebrain development; negative regulation of Notch signaling pathway; neuroblast division in the subventricular zone; positive regulation of dendrite morphogenesis; lateral ventricle development; positive regulation of cell migration