TrkB / NTRK2 recombinant protein

Recombinant Mouse TrkB / NTRK2 Protein (His tag)

Cat. Num. AAA2547142

• Gene Names: Ntrk2; Tkrb; trkB; trk-B; GP145-TrkB/GP95-TrkB
• Purity: > 98 % as determined by SDS-PAGE
• Synonyms: TrkB / NTRK2; Recombinant Mouse TrkB / NTRK2 Protein (His tag); AI848316; C030027L06Rik; Tkrb; trkB; TrkB / NTRK2 recombinant protein

• Host: Human Cells
• Purity/Purification: > 98 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 821

• Application Notes: The secreted recombinant mouse NTRK2 consists of 409amino acids and has a calculated molecular mass of 46 kDa. The apparent molecular mass of rm NTRK2 is approximately 75-85 kDa in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Cys 32
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

Related Product Information for TrkB / NTRK2 recombinant protein

Background: TrkB receptor also known as TrkB tyrosine kinase or BDNF/NT-3 growth factors receptor or neurotrophic tyrosine kinase, receptor, type 2 (NTRK2) is a single transmembrane catalytic receptors with intracellular tyrosine kinase activity. TrkB/NTRK2 is a member of the neurotrophic tyrosine receptor kinase (NTRK) family. TrkB tyrosine kinase (TrkB) or NTRK2 is coupled to the Ras, Cdc42/Rac/RhoG, MAPK, PI3-K and PLCgamma signaling pathways. There are four members of the Trk family; TrkA, TrkB and TrkC and a related p75NTR receptor. Each family member binds different neurotrophins with varying affinities. TrkB/NTRK has highest affinity for brain-derived neurotrophic factor (BDNF) and is involved in neuronal plasticity, longterm potentiation and apoptosis of CNS neurons. Other neurotrophins include nerve growth factor (NGF), neurotrophin-3 and neurotrophin-4. TrkB/NTRK is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. Signalling through this kinase leads to cell differentiation. Mutations in TrkB/NTRK have been associated with obesity and mood disorders.

Description: A DNA sequence encoding the extracellular domain of mouse NTRK2 (P15209-2) (Met 1-His 429) was expressed, with a C-terminal polyhistidine tag.

NCBI and Uniprot Product Information

• NCBI GI #: 68215970
• NCBI GeneID: 18212
• NCBI Accession #: NP_001020245.1
• NCBI GenBank Nucleotide #: NM_001025074.2
• UniProt Accession #: P15209; Q3TUF9; Q80WU0; Q91XJ9
• Molecular Weight: 83,744 Da
• NCBI Official Full Name: BDNF/NT-3 growth factors receptor isoform a
• NCBI Official Synonym Full Names: neurotrophic tyrosine kinase, receptor, type 2
• NCBI Official Symbol: Ntrk2
• NCBI Official Synonym Symbols: Tkrb; trkB; trk-B; GP145-TrkB/GP95-TrkB
• NCBI Protein Information: BDNF/NT-3 growth factors receptor
• UniProt Protein Name: BDNF/NT-3 growth factors receptor
• UniProt Gene Name: Ntrk2
• UniProt Synonym Gene Names: Trkb; Trk-B

Uniprot Description

TrkB: a receptor tyrosine kinase of the Trk family. Receptor for brain-derived neurotrophic factor (BDNF), neurotrophin-3, -4 and -5 but not nerve growth factor (NGF). Involved in the development and maintenance of the nervous system. Three splice variant isoforms have been described.

Protein type: EC 2.7.10.1; Kinase, protein; Membrane protein, integral; Protein kinase, TK; Protein kinase, tyrosine (receptor); TK group; Trk family

Chromosomal Location of Human Ortholog: 13 B1|13 31.2 cM

Cellular Component: axon; cell soma; cell surface; cytoplasm; cytosol; dendrite; dendritic spine; endosome; excitatory synapse; growth cone; integral to plasma membrane; nerve terminal; perikaryon; plasma membrane; postsynaptic density; postsynaptic membrane; presynaptic active zone; receptor complex; rough endoplasmic reticulum; terminal button

Molecular Function: brain-derived neurotrophic factor binding; brain-derived neurotrophic factor receptor activity; neurotrophin binding; neurotrophin receptor activity; protein binding; protein homodimerization activity; receptor tyrosine kinase binding; transmembrane receptor protein tyrosine kinase activity

Biological Process: brain-derived neurotrophic factor receptor signaling pathway; central nervous system neuron development; cerebral cortex development; circadian rhythm; feeding behavior; glutamate secretion; learning; long-term memory; mechanoreceptor differentiation; negative regulation of neuron apoptosis; neuromuscular junction development; neuron differentiation; neuron migration; oligodendrocyte differentiation; peripheral nervous system neuron development; positive regulation of axonogenesis; positive regulation of cell proliferation; positive regulation of MAPKKK cascade; positive regulation of peptidyl-serine phosphorylation; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of synaptic transmission, glutamatergic; positive regulation of synaptogenesis; protein amino acid autophosphorylation; regulation of dendrite development; regulation of GTPase activity; regulation of MAPKKK cascade; regulation of metabolic process; regulation of neurotransmitter secretion; regulation of protein kinase B signaling cascade; retina development in camera-type eye; retinal rod cell development; vasculogenesis