TrkB Active Protein | NTRK2 active protein

TrkB Protein, Human, Recombinant (His & hFc Tag)

Cat. Num. AAA8119962

• Gene Names: NTRK2; TRKB; trk-B; GP145-TrkB
• Purity: >90% as determined by SDS-PAGE
• Synonyms: TrkB; TrkB Protein; Human; Recombinant (His & hFc Tag); Human TrkB/NTRK2 Protein (His & Fc Tag); GP145-TrkB Protein; GP145-TrkB/GP95-TrkB Protein; Tkrb Protein; trk-B Protein; TRKB Protein; neurotrophic tyrosine kinase; receptor; type 2; NTRK2 active protein

• Host: HEK293 Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-His430

• Species: Human
• Activity: Measured by its ability to bind mouse BDNF in functional ELISA.
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Cys 32
• Tag: C-human IgG1-Fc & His
• Protein Construction: A DNA sequence encoding the extracellular domain (Met 1-His 430) of human TrkB (NP_001007098.1) precursor was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the carboxy-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Testing Data

(Measured by its ability to bind mouse BDNF in functional ELISA.)

Related Product Information for NTRK2 active protein

Background: TrkB receptor also known as TrkB tyrosine kinase or BDNF/NT-3 growth factors receptor or neurotrophic tyrosine kinase, receptor, type 2 (NTRK2) is a single transmembrane catalytic receptor with intracellular tyrosine kinase activity. TrkB/NTRK2 is a member of the neurotrophic tyrosine receptor kinase (NTRK) family. TrkB tyrosine kinase (TrkB) or NTRK2 is coupled to the Ras, Cdc42/Rac/RhoG, MAPK, PI3-K, and PLCgamma signaling pathways. There are four members of the Trk family; TrkA, TrkB, and TrkC and a related p75NTR receptor. Each family member binds different neurotrophins with varying affinities. TrkB/NTRK has the highest affinity for brain-derived neurotrophic factor (BDNF) and is involved in neuronal plasticity, long-term potentiation, and apoptosis of CNS neurons. Other neurotrophins include nerve growth factor(NGF), neurotrophin-3 and neurotrophin-4. TrkB/NTRK is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. Signaling through this kinase leads to cell differentiation. Mutations in TrkB/NTRK have been associated with obesity and mood disorders.

Product Categories/Family for NTRK2 active protein

Receptor Tyrosine Kinases (RTKs) Cancer Drug Targets

References

Klein R, et al. (1990) The trkB tyrosine protein kinase gene codes for a second neurogenic receptor that lacks the catalytic kinase domain. Cell. 61 (4): 647-56.
Rose CR, et al. (2003) Truncated TrkB-T1 mediates neurotrophin-evoked calcium signalling in glia cells. Nature. 426 (6962): 74-8.
Yamada K, et al. (2004) Brain-derived neurotrophic factor/TrkB signaling in memory processes. J Pharmacol Sci. 91 (4): 267-70.

NCBI and Uniprot Product Information

• NCBI GI #: 55956790
• NCBI GeneID: 4915
• NCBI Accession #: NP_001007098.1
• NCBI GenBank Nucleotide #: NM_001007097.1
• UniProt Accession #: Q16620; Q16675; Q59GJ1; Q8WXJ5; Q8WXJ6; Q8WXJ7; B1ANZ4; B4DFV9
• Molecular Weight: 91,999 Da
• NCBI Official Full Name: BDNF/NT-3 growth factors receptor isoform b
• NCBI Official Synonym Full Names: neurotrophic tyrosine kinase, receptor, type 2
• NCBI Official Symbol: NTRK2
• NCBI Official Synonym Symbols: TRKB; trk-B; GP145-TrkB
• NCBI Protein Information: BDNF/NT-3 growth factors receptor; trkB tyrosine kinase; tyrosine kinase receptor B; tropomyosin-related kinase B; BDNF-tropomyosine receptor kinase B
• UniProt Protein Name: BDNF/NT-3 growth factors receptor
• Protein Family: BDNF/NT-3 growth factors receptor
• UniProt Gene Name: NTRK2
• UniProt Synonym Gene Names: TRKB; Trk-B
• UniProt Entry Name: NTRK2_HUMAN

NCBI Description

This gene encodes a member of the neurotrophic tyrosine receptor kinase (NTRK) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. Signalling through this kinase leads to cell differentiation. Mutations in this gene have been associated with obesity and mood disorders. Alternate transcriptional splice variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

TrkB: a receptor tyrosine kinase of the Trk family. Receptor for brain-derived neurotrophic factor (BDNF), neurotrophin-3, -4 and -5 but not nerve growth factor (NGF). Involved in the development and maintenance of the nervous system. Three splice variant isoforms have been described.

Protein type: Membrane protein, integral; EC 2.7.10.1; Protein kinase, tyrosine (receptor); Protein kinase, TK; Kinase, protein; TK group; Trk family

Chromosomal Location of Human Ortholog: 9q22.1

Cellular Component: postsynaptic membrane; cell surface; growth cone; rough endoplasmic reticulum; integral to plasma membrane; dendritic spine; presynaptic active zone; excitatory synapse; terminal button; perikaryon; endosome membrane; cytosol; receptor complex

Molecular Function: brain-derived neurotrophic factor receptor activity; neurotrophin binding; protein homodimerization activity; ephrin receptor binding; brain-derived neurotrophic factor binding; ATP binding

Biological Process: circadian rhythm; mechanoreceptor differentiation; retinal rod cell development; peptidyl-tyrosine phosphorylation; nerve growth factor receptor signaling pathway; protein amino acid autophosphorylation; regulation of dendrite development; neuron migration; regulation of neurotransmitter secretion; positive regulation of synaptic transmission, glutamatergic; neuron differentiation; positive regulation of MAPKKK cascade; positive regulation of cell proliferation; oligodendrocyte differentiation; feeding behavior; negative regulation of neuron apoptosis; inflammatory response; vasculogenesis; neuromuscular junction development; central nervous system neuron development; aging; response to light stimulus; brain-derived neurotrophic factor receptor signaling pathway; adenylate cyclase activation; regulation of protein kinase B signaling cascade; learning; positive regulation of peptidyl-serine phosphorylation; positive regulation of phosphoinositide 3-kinase cascade; peripheral nervous system neuron development; long-term memory; glutamate secretion; positive regulation of axonogenesis; cerebral cortex development; transmembrane receptor protein tyrosine kinase signaling pathway

Disease: Obesity, Hyperphagia, And Developmental Delay