Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Rat CD40 expression on rat splenocytes. SD splenocytes were stained with PE-conjugated anti-Rat CD40 and FITC conjugated anti-Rat CD3 (BD Pharmingen'). The dot plots were derived from events with the forward and side light-scatter characteristics of intact cells.)
Mouse anti-Rat CD40 Monoclonal Antibody | anti-CD40 antibody
Anti-CD40 Antibody (PE), Mouse Monoclonal
Gene Names
Cd40; Tnfrsf5
Reactivity
Rat
Applications
Flow Cytometry, Functional Assay
Purity
Protein A
Synonyms
CD40; Monoclonal Antibody; Anti-CD40 Antibody (PE); Mouse Monoclonal; CD40 Antibody (PE); Mouse MAb; CD40 molecule; TNF receptor superfamily member 5; anti-CD40 antibody
Host
Mouse
Reactivity
Rat
Clonality
Monoclonal
Isotype
Mouse IgG1
Clone Number
01
Specificity
Rat CD40/TNFRSF5
Purity/Purification
Protein A
Form/Format
Liquid; Aqueous solution containing 0.5% BSA and 0.09% sodium azide
Concentration
5ul/Test, 0.1mg/ml (varies by lot)
Applicable Applications for anti-CD40 antibody
Flow Cytometry (FC/FACS/FCM)
Immunogen
Recombinant Rat CD40/TNFRSF5 protein
Conjugation
PE
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Rat CD40/TNFRSF5 (rR CD40/TNFRSF5) and conjugated with PE under optimum conditions, the unreacted PE was removed.
Preparation and Storage
This antibody can be stored at 2-8 degree C for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Rat CD40 expression on rat splenocytes. SD splenocytes were stained with PE-conjugated anti-Rat CD40 and FITC conjugated anti-Rat CD3 (BD Pharmingen'). The dot plots were derived from events with the forward and side light-scatter characteristics of intact cells.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Rat CD40 expression on rat splenocytes. SD splenocytes were stained with PE-conjugated anti-Rat CD40 and FITC conjugated anti-Rat CD3 (BD Pharmingen'). The dot plots were derived from events with the forward and side light-scatter characteristics of intact cells.)
Related Product Information for anti-CD40 antibody
CD40, also known as TNFRSF5, is a member of the TNF receptor superfamily which are single transmembrane-spanning glycoproteins. CD40 protein plays an essential role in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. CD40 protein is expressed in B cells, dendritic cells, macrophages, endothelial cells, and several tumor cell lines. Defects in CD40 result in hyper-IgM immunodeficiency type 3 (HIGM3). In addition, CD40/CD40L interaction is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis.
Product Categories/Family for anti-CD40 antibody
References
van Kooten C, et al. (2000). CD40-CD40 ligand. J Leukoc Biol. 67 (1): 2-17.Bhushan A, et al. (2002). CD40:CD40L interactions in X-linked and non-X-linked hyper-IgM syndromes. Immunol Res. 24 (3): 311-24.Chatzigeorgiou A, et al. (2009) CD40/CD40L signaling and its implication in health and disease. Biofactors. 35(6): 474-83.Li R, et al. (2009) Expression of CD40 and CD40L in Gastric Cancer Tissue and Its Clinical Significance. Int J Mol Sci. 10(9): 3900-17.Lievens D, et al. (2009) The multi-functionality of CD40L and its receptor CD40 in atherosclerosis. Thromb Haemost. 102(2): 206-14.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
31,801 Da
NCBI Official Full Name
tumor necrosis factor receptor superfamily member 5
NCBI Official Synonym Full Names
CD40 molecule
NCBI Official Symbol
Cd40
NCBI Official Synonym Symbols
Tnfrsf5
NCBI Protein Information
tumor necrosis factor receptor superfamily member 5
UniProt Protein Name
CD40 molecule, TNF receptor superfamily member 5
Protein Family
UniProt Gene Name
Cd40
NCBI Description
may mediate chronic inflammation including arteriosclerosis; interacts with ligand CD154 and plays a crucial role in humoral and cellular immunity, and in T-cell-mediated inflammatory responses [RGD, Feb 2006]
Research Articles on CD40
Similar Products
Product Notes
The CD40 cd40 (Catalog #AAA8112778) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD40 Antibody (PE), Mouse Monoclonal reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CD40 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS/FCM). Researchers should empirically determine the suitability of the CD40 cd40 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD40, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
