Typical Testing Data/Standard Curve (for reference only)
(Human TrkB PicoKine ELISA Kit standard curve )
Human TrkB ELISA Kit | NTRK2 elisa kit
Human TrkB PicoKine ELISA Kit
O.D.: |0.009|0.084|0.149|0.269|0.459|0.818|1.530|2.212
n: |16|16|16|24|24|24
Mean(pg/ml): |202|1009|4057|195|887|3332
Standard Deviation: |12.72|54.48|198.73|14.62|54.10|193.25
CV(%): |6.3|5.4|4.9|7.5|6.1|5.8
Typical Testing Data/Standard Curve (for reference only)
(Human TrkB PicoKine ELISA Kit standard curve )
Typical Testing Data/Standard Curve (for reference only)
(Human TrkB PicoKine ELISA Kit standard curve )
Background: TrkB receptor also known as TrkB tyrosine kinase or BDNF/NT-3 growth factors receptor or neurotrophic tyrosine kinase, receptor, type 2 is a protein that in humans is encoded by the NTRK2 gene.[1] TrkB is the high affinity catalytic receptor for several "neurotrophins", which are small protein growth factors that induce the survival and differentiation of distinct cell populations. The TrkB receptor is part of the large family of receptor tyrosine kinases. A "tyrosine kinase" is an enzyme which is capable of adding a phosphate group to certain tyrosines on target proteins, or "substrates".Soppet et al. [2] demonstrated that the gp145 gene product of the TRKB gene is rapidly phosphorylated on tyrosine residues upon exposure to BDNF and NTF3.
Principle: Human TrkB ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from Mouse specific for TrkB has been precoated onto 96-well plates. Standards (Expression system for standard: NSO, Immunogen sequence: C32-H430) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TrkB is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substRate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human TrkB amount of sample captured in plate.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the neurotrophic tyrosine receptor kinase (NTRK) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. Signalling through this kinase leads to cell differentiation. Mutations in this gene have been associated with obesity and mood disorders. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]
Uniprot Description
TrkB: a receptor tyrosine kinase of the Trk family. Receptor for brain-derived neurotrophic factor (BDNF), neurotrophin-3, -4 and -5 but not nerve growth factor (NGF). Involved in the development and maintenance of the nervous system. Three splice variant isoforms have been described.
Protein type: EC 2.7.10.1; Kinase, protein; Membrane protein, integral; Protein kinase, TK; Protein kinase, tyrosine (receptor); TK group; Trk family
Chromosomal Location of Human Ortholog: 9q21.33
Cellular Component: integral to plasma membrane; receptor complex
Molecular Function: brain-derived neurotrophic factor binding; brain-derived neurotrophic factor receptor activity; neurotrophin binding; protein homodimerization activity
Biological Process: brain-derived neurotrophic factor receptor signaling pathway; central nervous system neuron development; cerebral cortex development; learning; negative regulation of neuron apoptosis; neuron differentiation; neuron migration; positive regulation of axonogenesis; positive regulation of cell proliferation; positive regulation of MAPKKK cascade; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein amino acid phosphorylation; protein amino acid autophosphorylation; regulation of GTPase activity
Disease: Obesity, Hyperphagia, And Developmental Delay
Research Articles on NTRK2
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Product Notes
The Human NTRK2 ntrk2 (Catalog #AAA178721) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA178721 ELISA Kit recognizes Human NTRK2. It is sometimes possible for the material contained within the vial of "TrkB, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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