Western Blot (WB)
( All lanes : Anti-GIGYF1 Antibody (Center) at 1:2000 dilution Lane 1: Ramos whole cell lysate Lane 2: Jurkat whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution. Predicted band size : 115 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit GIGYF1 Polyclonal Antibody | anti-GIGYF1 antibody
GIGYF1 Antibody (Center)
Gene Names
GIGYF1; GYF1; PERQ1
Reactivity
HumanPredicted Reactivity: Mouse
Applications
Western Blot, ELISA
Synonyms
GIGYF1; Polyclonal Antibody; GIGYF1 Antibody (Center); PERQ amino acid-rich with GYF domain-containing protein 1; GRB10-interacting GYF protein 1; CDS2; PERQ1; anti-GIGYF1 antibody
Host
Rabbit
Reactivity
Human
Predicted Reactivity: Mouse
Predicted Reactivity: Mouse
Clonality
Polyclonal
Isotype
Ig
Specificity
This GIGYF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 666-694 amino acids from the Central region of human GIGYF1.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Positions
666-694
Applicable Applications for anti-GIGYF1 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB~~1:2000
Function
May act cooperatively with GRB10 to regulate tyrosine kinase receptor signaling. May increase IGF1 receptor phosphorylation under IGF1 stimulation as well as phosphorylation of IRS1 and SHC1 (By similarity).
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
( All lanes : Anti-GIGYF1 Antibody (Center) at 1:2000 dilution Lane 1: Ramos whole cell lysate Lane 2: Jurkat whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution. Predicted band size : 115 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
( All lanes : Anti-GIGYF1 Antibody (Center) at 1:2000 dilution Lane 1: Ramos whole cell lysate Lane 2: Jurkat whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution. Predicted band size : 115 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-GIGYF1 antibody
May act cooperatively with GRB10 to regulate tyrosine kinase receptor signaling. May increase IGF1 receptor phosphorylation under IGF1 stimulation as well as phosphorylation of IRS1 and SHC1 (By similarity).
Product Categories/Family for anti-GIGYF1 antibody
References
Szafranski, K., et al. Genome Biol. 8 (8), R154 (2007) :
Beausoleil, S.A., et al. Nat. Biotechnol. 24(10):1285-1292(2006)
Giovannone, B., et al. J. Biol. Chem. 278(34):31564-31573(2003)
Wilson, M.D., et al. Nucleic Acids Res. 29(6):1352-1365(2001)
Glockner, G., et al. Genome Res. 8(10):1060-1073(1998)
Beausoleil, S.A., et al. Nat. Biotechnol. 24(10):1285-1292(2006)
Giovannone, B., et al. J. Biol. Chem. 278(34):31564-31573(2003)
Wilson, M.D., et al. Nucleic Acids Res. 29(6):1352-1365(2001)
Glockner, G., et al. Genome Res. 8(10):1060-1073(1998)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Calculated MW: 114601
NCBI Official Full Name
PERQ amino acid-rich with GYF domain-containing protein 1
NCBI Official Synonym Full Names
GRB10 interacting GYF protein 1
NCBI Official Symbol
GIGYF1
NCBI Official Synonym Symbols
GYF1; PERQ1
NCBI Protein Information
PERQ amino acid-rich with GYF domain-containing protein 1
UniProt Protein Name
PERQ amino acid-rich with GYF domain-containing protein 1
Protein Family
UniProt Gene Name
GIGYF1
UniProt Synonym Gene Names
CDS2; PERQ1
UniProt Entry Name
PERQ1_HUMAN
Uniprot Description
PERQ1: May act cooperatively with GRB10 to regulate tyrosine kinase receptor signaling. May increase IGF1 receptor phosphorylation under IGF1 stimulation as well as phosphorylation of IRS1 and SHC1. Belongs to the PERQ family.
Protein type: Unknown function
Chromosomal Location of Human Ortholog: 7q22
Biological Process: insulin-like growth factor receptor signaling pathway
Research Articles on GIGYF1
Similar Products
Product Notes
The GIGYF1 gigyf1 (Catalog #AAA9208678) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 666-694. The GIGYF1 Antibody (Center) reacts with Human Predicted Reactivity: Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's GIGYF1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:2000. Researchers should empirically determine the suitability of the GIGYF1 gigyf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GIGYF1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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