TrkB antibody kit

TrkB Antibody Sampler Kit

Cat. Num. AAA2550097

• Gene Names: NTRK2; OBHD; TRKB; trk-B; GP145-TrkB
• Applications: Western Blot
• Synonyms: TrkB; TrkB Antibody Sampler Kit; TrkB antibody kit

• Sequence Length: 822

• Applicable Applications for TrkB antibody kit: Western Blot (WB)

Western Blot (WB)

(Western Blot analysis of HepG2 cells using Trk B Polyclonal Antibody at dilution of 1:500.)

Western Blot (WB)

(Western Blot analysis of Rat muscle with Phospho-Trk B (Tyr516) Polyclonal Antibody at dilution of 1:1000)

Western Blot (WB)

(Western Blot analysis of 293T cells with Phospho-Trk B (Tyr706) Polyclonal Antibody at dilution of 1:1000)

Related Product Information for TrkB antibody kit

Background: The family of Trk receptor tyrosine kinases consists of TrkA, TrkB, and TrkC. While the sequence of these family members is highly conserved, they are activated by different neurotrophins: TrkA by NGF, TrkB by BDNF or NT4, and TrkC by NT3. Neurotrophin signaling through these receptors regulates a number of physiological processes, such as cell survival, proliferation, neural development, and axon and dendrite growth and patterning. In the adult nervous system, the Trk receptors regulate synaptic strength and plasticity. TrkA regulates proliferation and is important for development and maturation of the nervous system. Phosphorylation at Tyr490 is required for Shc association and activation of the Ras-MAP kinase cascade. Residues Tyr674/675 lie within the catalytic domain, and phosphorylation at these sites reflects TrkA kinase activity. Point mutations, deletions, and chromosomal rearrangements (chimeras) cause ligand-independent receptor dimerization and activation of TrkA. TrkA is activated in many malignancies including breast, ovarian, prostate, and thyroid carcinomas. Research studies suggest that expression of TrkA in neuroblastomas may be a good prognostic marker as TrkA signals growth arrest and differentiation of cells originating from the neural crest. The phosphorylation sites are conserved between TrkA and TrkB: Tyr490 of TrkA corresponds to Tyr512 in TrkB, and Tyr674/675 of TrkA to Tyr706/707 in TrkB of the human sequence. TrkB is overexpressed in tumors, such as neuroblastoma, prostate adenocarcinoma, and pancreatic ductal adenocarcinoma. Research studies have shown that in neuroblastomas, overexpression of TrkB correlates with an unfavorable disease outcome when autocrine loops signaling tumor survival are potentiated by additional overexpression of brain- derived neurotrophic factor (BDNF). An alternatively spliced truncated TrkB isoform lacking the kinase domain is overexpressed in Wilms' tumors and this isoform may act as a dominant-negative regulator of TrkB signaling.

NCBI and Uniprot Product Information

• NCBI GI #: 913718
• NCBI GeneID: 4915
• Molecular Weight: 35,332 Da
• NCBI Official Full Name: trkB
• NCBI Official Synonym Full Names: neurotrophic receptor tyrosine kinase 2
• NCBI Official Symbol: NTRK2
• NCBI Official Synonym Symbols: OBHD; TRKB; trk-B; GP145-TrkB
• NCBI Protein Information: BDNF/NT-3 growth factors receptor
• UniProt Protein Name: BDNF/NT-3 growth factors receptor
• UniProt Gene Name: NTRK2
• UniProt Synonym Gene Names: TRKB; Trk-B

NCBI Description

This gene encodes a member of the neurotrophic tyrosine receptor kinase (NTRK) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. Signalling through this kinase leads to cell differentiation. Mutations in this gene have been associated with obesity and mood disorders. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]

Uniprot Description

TrkB: a receptor tyrosine kinase of the Trk family. Receptor for brain-derived neurotrophic factor (BDNF), neurotrophin-3, -4 and -5 but not nerve growth factor (NGF). Involved in the development and maintenance of the nervous system. Three splice variant isoforms have been described.

Protein type: EC 2.7.10.1; Kinase, protein; Membrane protein, integral; Protein kinase, TK; Protein kinase, tyrosine (receptor); TK group; Trk family

Chromosomal Location of Human Ortholog: 9q21.33

Cellular Component: cytosol; early endosome; early endosome membrane; integral component of plasma membrane; postsynaptic density; postsynaptic membrane; receptor complex; terminal bouton

Molecular Function: ATP binding; brain-derived neurotrophic factor binding; brain-derived neurotrophic factor-activated receptor activity; neurotrophin binding; protein binding; protein homodimerization activity

Biological Process: brain-derived neurotrophic factor receptor signaling pathway; central nervous system neuron development; cerebral cortex development; circadian rhythm; feeding behavior; glutamate secretion; learning; mechanoreceptor differentiation; negative regulation of anoikis; negative regulation of neuron apoptosis; neuromuscular junction development; neuron differentiation; neuron migration; neurotrophin signaling pathway; oligodendrocyte differentiation; peptidyl-tyrosine phosphorylation; peripheral nervous system neuron development; positive regulation of axonogenesis; positive regulation of cell proliferation; positive regulation of gene expression; positive regulation of MAPK cascade; positive regulation of neuron projection development; positive regulation of peptidyl-serine phosphorylation; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein phosphorylation; positive regulation of synaptogenesis; protein autophosphorylation; regulation of GTPase activity; regulation of protein kinase B signaling; retina development in camera-type eye; retinal rod cell development; vasculogenesis

Disease: Obesity, Hyperphagia, And Developmental Delay