TrkA / NTRK1 recombinant protein

Recombinant Human TrkA / NTRK1 Protein (His & Fc tag)

Cat. Num. AAA2545937

• Gene Names: NTRK1; MTC; TRK; TRK1; TRKA; Trk-A; p140-TrkA
• Purity: > 98 % as determined by SDS-PAGE
• Synonyms: TrkA / NTRK1; Recombinant Human TrkA / NTRK1 Protein (His & Fc tag); DKFZp781I14186; MTC; TRK; TRK1; TRKA; p140-TrkA; NTRK1; TrkA / NTRK1 recombinant protein

• Host: Human Cells
• Purity/Purification: > 98 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 796

• Application Notes: The recombinant human NTRK1/Fc is a disulfide-linked homodimer. The reduced monomer consists of 598 amino acids and has a predicted molecular mass of 66 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh NTRK1/Fc monomer is approximately 85-95 kDa due to glycosylation.
• Predicted N Terminal: Ala 33
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for TrkA / NTRK1 recombinant protein

Background: TRKA is a member of the neurotrophic tyrosine kinase receptor (NTKR) family. It is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. Isoform TrkA-III promotes angiogenesis and has oncogenic activity when overexpressed. Isoform TrkA-I is found in most non-neuronal tissues. Isoform TrkA-II is primarily expressed in neuronal cells. TrkA-III is specifically expressed by pluripotent neural stem and neural crest progenitors. The presence of NTRK1 leads to cell differentiation and may play a role in specifying sensory neuron subtype s. Mutations in TRKA gene have been associated with congenital insensitivity to pain, anhidrosis, self-mutilating behavior, mental retardation and cancer. It was originally identified as an oncogene as it is commonly mutated in cancers, particularly colon and thyroid carcinomas. TRKA is required for high-affinity binding to nerve growth factor (NGF), neurotrophin-3 and neurotrophin-4/5 but not brain-derived neurotrophic factor (BDNF). Known substrates for the Trk receptors are SHC1, PI 3-kinase, and PLC-gamma-1. NTRK1 has a crucial role in the development and function of the nociceptive reception system as well as establishment of thermal regulation via sweating. It also activates ERK1 by either SHC1- or PLC-gamma-1-dependent signaling pathway. Defects in NTRK1 are a cause of congenital insensitivity to pain with anhidrosis and thyroid papillary carcinoma.

Description: A DNA sequence encoding the human NTRK1 (NP_002520.2) extracellular domain (Met 1-Pro 382) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 4585712
• NCBI GeneID: 4914
• NCBI Accession #: NP_002520.2
• NCBI GenBank Nucleotide #: NM_002529.3
• UniProt Accession #: P04629; P08119; Q15655; Q15656; Q5D056; Q5VZS2; Q7Z5C3; Q9UIU7; B2R6T5; B7ZM34
• Molecular Weight: 77,145 Da
• NCBI Official Full Name: high affinity nerve growth factor receptor isoform 2
• NCBI Official Synonym Full Names: neurotrophic receptor tyrosine kinase 1
• NCBI Official Symbol: NTRK1
• NCBI Official Synonym Symbols: MTC; TRK; TRK1; TRKA; Trk-A; p140-TrkA
• NCBI Protein Information: high affinity nerve growth factor receptor
• UniProt Protein Name: High affinity nerve growth factor receptor
• UniProt Gene Name: NTRK1
• UniProt Synonym Gene Names: MTC; TRK; TRKA; Trk-A

NCBI Description

This gene encodes a member of the neurotrophic tyrosine kinase receptor (NTKR) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. The presence of this kinase leads to cell differentiation and may play a role in specifying sensory neuron subtypes. Mutations in this gene have been associated with congenital insensitivity to pain, anhidrosis, self-mutilating behavior, mental retardation and cancer. Alternate transcriptional splice variants of this gene have been found, but only three have been characterized to date. [provided by RefSeq, Jul 2008]

Uniprot Description

TrkA: a receptor tyrosine kinase of the Trk family. High affinity receptor for nerve growth factor, neurotrophin-3 and neurotrophin-4/5 but not brain- derived neurotrophic factor. Known substrates include Shc, PI-3K, and PLC-gamma-1. Has a crucial role in the development and function of the nociceptive reception system. Two splice variant isoforms have been described.

Protein type: EC 2.7.10.1; Kinase, protein; Membrane protein, integral; Oncoprotein; Protein kinase, TK; Protein kinase, tyrosine (receptor); TK group; Trk family

Chromosomal Location of Human Ortholog: 1q23.1

Cellular Component: early endosome; endosome; integral to plasma membrane; late endosome; plasma membrane; protein complex; receptor complex

Molecular Function: nerve growth factor binding; nerve growth factor receptor activity; neurotrophin binding; protein binding; protein homodimerization activity; transmembrane receptor protein tyrosine kinase activity

Biological Process: activation of MAPKK activity; activation of NF-kappaB transcription factor; microtubule-based movement; negative regulation of cell proliferation; negative regulation of neuron apoptosis; nerve growth factor receptor signaling pathway; phosphoinositide-mediated signaling; positive regulation of GTPase activity; positive regulation of programmed cell death; positive regulation of protein amino acid phosphorylation; positive regulation of Ras protein signal transduction; protein amino acid autophosphorylation; protein amino acid phosphorylation; sympathetic nervous system development

Disease: Insensitivity To Pain, Congenital, With Anhidrosis; Thyroid Carcinoma, Familial Medullary