TrkA Active Protein | NTRK1 active protein

TrkA Protein, Human, Recombinant (aa 285-413, His Tag)

Cat. Num. AAA8120671

• Gene Names: NTRK1; MTC; TRK; TRK1; TRKA; Trk-A; p140-TrkA
• Purity: >97% as determined by SDS-PAGE
• Synonyms: TrkA; TrkA Protein; Human; Recombinant (aa 285-413; His Tag); Human TrkA/NTRK1 Protein (aa 285-413; MTC Protein; p140-TrkA Protein; TRK Protein; Trk-A Protein; TRK1 Protein; TRKA Protein; neurotrophic tyrosine kinase; receptor; type 1; NTRK1 active protein

• Host: E Coli
• Purity/Purification: >97% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 50mM Tirs, 200mM NaCl, pH8.0. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Pro285-Glu413

• Species: Human
• Activity: Measured by its ability to inhibit NGF-induced proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.2-0.8ug/ml in the presence of 10ng/mL of Recombinant human NGF.
• Predicted N Terminal: Met
• Tag: N-His
• Protein Construction: A DNA sequence encoding the amino acid sequence (Pro 285-Glu 413) of human NTRK1 (NP_002520.2), corresponding to the Ig-like C2-type 2 domain, was expressed and purified, with a N-terminal polyhistidine tag.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Testing Data

(Measured by its ability to inhibit NGF-induced proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.2-0.8 ug/ml in the presence of 10 ng/mL of Recombinant human NGF.)

Related Product Information for NTRK1 active protein

Background: TRKA is a member of the neurotrophic tyrosine kinase receptor (NTKR) family. It is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. Isoform TrkA-III promotes angiogenesis and has oncogenic activity when overexpressed. Isoform TrkA-I is found in most non-neuronal tissues. Isoform TrkA-II is primarily expressed in neuronal cells. TrkA-III is specifically expressed by the pluripotent neural stem and neural crest progenitors. The presence of NTRK1 leads to cell differentiation and may play a role in specifying sensory neuron subtypes. Mutations in the TRKA gene have been associated with congenital insensitivity to pain, anhidrosis, self-mutilating behavior, mental retardation, and cancer. It was originally identified as an oncogene as it is commonly mutated in cancers, particularly colon and thyroid carcinomas. TRKA is required for high-affinity binding to nerve growth factor (NGF), neurotrophin-3 and neurotrophin-4/5 but not brain-derived neurotrophic factor (BDNF). Known substrates for the Trk receptors are SHC1, PI 3-kinase, and PLC-gamma-1. NTRK1 has a crucial role in the development and function of the nociceptive reception system as well as the establishment of thermal regulation via sweating. It also activates ERK1 by either SHC1- or PLC-gamma-1-dependent signaling pathway. Defects in NTRK1 are a cause of congenital insensitivity to pain with anhidrosis and thyroid papillary carcinoma.

Product Categories/Family for NTRK1 active protein

Cancer Drug Targets Receptor Tyrosine Kinases (RTKs)

References

Lambiase A, et al. (2005) Molecular basis for keratoconus: lack of TrkA expression and its transcriptional repression by Sp3. Natl Acad Sci. 102 (46):16795-800.
Benito-Gutia©rrez E, et al. (2006) Origin and evolution of the Trk family of neurotrophic receptors. Mol Cell Neurosci. 31(2):179-92.
Martin-Zanca D, et al. (1986) A human oncogene formed by the fusion of truncated tropomyosin and protein tyrosine kinase sequences. Nature. 319(6056):743-8.

NCBI and Uniprot Product Information

• NCBI GI #: 4585712
• NCBI GeneID: 4914
• NCBI Accession #: NP_002520.2
• NCBI GenBank Nucleotide #: NM_002529.3
• UniProt Accession #: P04629; P08119; Q15655; Q15656; Q5D056; Q5VZS2; Q7Z5C3; Q9UIU7; B2R6T5; B7ZM34
• Molecular Weight: 77,145 Da
• NCBI Official Full Name: high affinity nerve growth factor receptor isoform 2
• NCBI Official Synonym Full Names: neurotrophic tyrosine kinase, receptor, type 1
• NCBI Official Symbol: NTRK1
• NCBI Official Synonym Symbols: MTC; TRK; TRK1; TRKA; Trk-A; p140-TrkA
• NCBI Protein Information: high affinity nerve growth factor receptor; gp140trk; Oncogene TRK; tyrosine kinase receptor A; tropomyosin-related kinase A; TRK1-transforming tyrosine kinase protein
• UniProt Protein Name: High affinity nerve growth factor receptor
• Protein Family: High affinity nerve growth factor receptor
• UniProt Gene Name: NTRK1
• UniProt Synonym Gene Names: MTC; TRK; TRKA; Trk-A
• UniProt Entry Name: NTRK1_HUMAN

NCBI Description

This gene encodes a member of the neurotrophic tyrosine kinase receptor (NTKR) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. The presence of this kinase leads to cell differentiation and may play a role in specifying sensory neuron subtypes. Mutations in this gene have been associated with congenital insensitivity to pain, anhidrosis, self-mutilating behavior, mental retardation and cancer. Alternate transcriptional splice variants of this gene have been found, but only three have been characterized to date. [provided by RefSeq, Jul 2008]

Uniprot Description

TrkA: a receptor tyrosine kinase of the Trk family. High affinity receptor for nerve growth factor, neurotrophin-3 and neurotrophin-4/5 but not brain- derived neurotrophic factor. Known substrates include Shc, PI-3K, and PLC-gamma-1. Has a crucial role in the development and function of the nociceptive reception system. Two splice variant isoforms have been described.

Protein type: Protein kinase, TK; Oncoprotein; EC 2.7.10.1; Membrane protein, integral; Protein kinase, tyrosine (receptor); Kinase, protein; TK group; Trk family

Chromosomal Location of Human Ortholog: 1q21-q22

Cellular Component: protein complex; cell surface; integral to plasma membrane; late endosome membrane; dendrite; early endosome; cell soma; early endosome membrane; axon; late endosome; plasma membrane; cytoplasmic vesicle; receptor complex; endosome

Molecular Function: neurotrophin p75 receptor binding; neurotrophin binding; protein binding; nerve growth factor receptor activity; protein homodimerization activity; ephrin receptor binding; nerve growth factor binding; transmembrane receptor protein tyrosine kinase activity; ATP binding

Biological Process: circadian rhythm; axon guidance; mechanoreceptor differentiation; peptidyl-tyrosine phosphorylation; activation of MAPKK activity; nerve growth factor receptor signaling pathway; protein amino acid autophosphorylation; positive regulation of synaptic transmission, glutamatergic; protein amino acid phosphorylation; olfactory nerve development; activation of NF-kappaB transcription factor; negative regulation of cell proliferation; sympathetic nervous system development; response to radiation; learning and/or memory; small GTPase mediated signal transduction; response to axon injury; negative regulation of neuron apoptosis; response to electrical stimulus; detection of temperature stimulus involved in sensory perception of pain; response to hydrostatic pressure; aging; response to drug; response to nutrient levels; phosphoinositide-mediated signaling; adenylate cyclase activation; Sertoli cell development; detection of mechanical stimulus involved in sensory perception of pain; positive regulation of programmed cell death; positive regulation of angiogenesis; response to ethanol; phospholipase C activation; B cell differentiation; Ras protein signal transduction; response to activity; positive regulation of Ras protein signal transduction; transmembrane receptor protein tyrosine kinase signaling pathway

Disease: Thyroid Carcinoma, Familial Medullary; Insensitivity To Pain, Congenital, With Anhidrosis