Western Blot (WB)
(Western blot detection of Nucleophosmin in HaCat, COS7, 293, HepG2 and Hela cell lysates using NPM1 mouse mAb (dilution 1:500).Predicted band size:33 Kda.Observed band size:38KDa.)
Nucleophosmin Monoclonal Antibody | anti-NPM antibody
Anti-Nucleophosmin
Source: Human
ICC: 1:100
Western Blot (WB)
(Western blot detection of Nucleophosmin in HaCat, COS7, 293, HepG2 and Hela cell lysates using NPM1 mouse mAb (dilution 1:500).Predicted band size:33 Kda.Observed band size:38KDa.)
Western Blot (WB)
(Western blot detection of Nucleophosmin in HaCat, COS7, 293, HepG2 and Hela cell lysates using NPM1 mouse mAb (dilution 1:500).Predicted band size:33 Kda.Observed band size:38KDa.)
Immunocytochemistry (ICC)
(Immunocytochemistry of HeLa cells using Nucleophosmin mouse mAb (dilution 1:100).)
Immunocytochemistry (ICC)
(Immunocytochemistry of HeLa cells using Nucleophosmin mouse mAb (dilution 1:100).)
Immunoprecipitation (IP)
(Immunoprecipitation analysis of Hela cell lysates using Nucleophosmin mouse mAb.)
Immunoprecipitation (IP)
(Immunoprecipitation analysis of Hela cell lysates using Nucleophosmin mouse mAb.)
UniPort Summary Function: Involved in diverse cellular processes such as ribosome biogenesis, centrosome duplication, protein chaperoning, histone assembly, cell proliferation, and regulation of tumor suppressors p53/TP53 and ARF. Binds ribosome presumably to drive ribosome nuclear export. Associated with nucleolar ribonucleoprotein structures and bind single-stranded nucleic acids. Acts as a chaperonin for the core histones H3, H2B and H4. Stimulates APEX1 endonuclease activity on apurinic/apyrimidinic (AP) double-stranded DNA but inhibits APEX1 endonuclease activity on AP single-stranded RNA. May exert a control of APEX1 endonuclease activity within nucleoli devoted to repair AP on rDNA and the removal of oxidized rRNA molecules. In concert with BRCA2, regulates centrosome duplication. Regulates centriole duplication: phosphorylation by PLK2 is able to trigger centriole replication. Negatively regulates the activation of EIF2AK2/PKR and suppresses apoptosis through inhibition of EIF2AK2/PKR autophosphorylation. Antagonizes the inhibitory effect of ATF5 on cell proliferation and relieves ATF5-induced G2/M blockade (PubMed:22528486).
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a phosphoprotein which moves between the nucleus and the cytoplasm. The gene product is thought to be involved in several processes including regulation of the ARF/p53 pathway. A number of genes are fusion partners have been characterized, in particular the anaplastic lymphoma kinase gene on chromosome 2. Mutations in this gene are associated with acute myeloid leukemia. More than a dozen pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Nov 2009]
Uniprot Description
NPM1: a nucleolar protein associated with nucleolar ribonucleoprotein structures and that binds single-stranded nucleic acids. Is a major component of template activating factor (TAF)-III. It plays a role in controlling centrosome duplication, and may function in the assembly and/or transport of the ribosome. Two alternatively spliced isoforms have been described.
Protein type: Nucleolus; Oncoprotein; RNA-binding; Translation
Chromosomal Location of Human Ortholog: 5q35.1
Cellular Component: centrosome; cytoplasm; cytosol; focal adhesion; membrane; nucleolus; nucleoplasm; nucleus; ribonucleoprotein complex; spindle pole centrosome
Molecular Function: histone binding; NF-kappaB binding; protein binding; protein heterodimerization activity; protein homodimerization activity; protein kinase binding; protein kinase inhibitor activity; ribosomal large subunit binding; ribosomal small subunit binding; RNA binding; Tat protein binding; transcription coactivator activity; unfolded protein binding
Biological Process: activation of NF-kappaB transcription factor; cell aging; centrosome cycle; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; DNA repair; DNA replication-independent nucleosome assembly at centromere; intracellular protein transport; negative regulation of apoptosis; negative regulation of cell proliferation; nucleocytoplasmic transport; nucleosome assembly; positive regulation of cell proliferation; positive regulation of transcription, DNA-dependent; positive regulation of translation; protein localization; protein oligomerization; regulation of centriole replication; regulation of endodeoxyribonuclease activity; response to stress; ribosome assembly
Research Articles on NPM
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Product Notes
The NPM npm1 (Catalog #AAA475607) is an Antibody produced from Mouse Source: Human and is intended for research purposes only. The product is available for immediate purchase. The Anti-Nucleophosmin reacts with Human, Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's Nucleophosmin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunoprecipitation (IP). WB: 1:500 ICC: 1:100. Researchers should empirically determine the suitability of the NPM npm1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Nucleophosmin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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