Western Blot (WB)
((0. 01ug/ml) staining of human kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat GST3/GSTP1 Polyclonal Antibody | anti-GSTP1 antibody
Goat anti-GST3/GSTP1 Antibody
Western Blot: Approx 24kDa band observed in Human Kidney, Liver and Lung lysates (calculated MW of 23.4kDa according to NP_000843.1). In transfected HEK293 transiently expressing GSTP1 band of approx.26 kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.01-0.03ug/ml.
Western Blot (WB)
((0. 01ug/ml) staining of human kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0. 01ug/ml) staining of human kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
(HEK293 overexpressing GSTP1 (RC203086) and probed (mock transfection in first lane), tested by Origene.)
Western Blot (WB)
(HEK293 overexpressing GSTP1 (RC203086) and probed (mock transfection in first lane), tested by Origene.)
NCBI and Uniprot Product Information
NCBI Description
Glutathione S-transferases (GSTs) are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. Based on their biochemical, immunologic, and structural properties, the soluble GSTs are categorized into 4 main classes: alpha, mu, pi, and theta. This GST family member is a polymorphic gene encoding active, functionally different GSTP1 variant proteins that are thought to function in xenobiotic metabolism and play a role in susceptibility to cancer, and other diseases. [provided by RefSeq, Jul 2008]
Uniprot Description
GSTP1: Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Regulates negatively CDK5 activity via p25/p35 translocation to prevent neurodegeneration. Homodimer. Interacts with CDK5. Belongs to the GST superfamily. Pi family.
Protein type: Xenobiotic Metabolism - drug metabolism - cytochrome P450; Transferase; Xenobiotic Metabolism - metabolism by cytochrome P450; Other Amino Acids Metabolism - glutathione; EC 2.5.1.18
Chromosomal Location of Human Ortholog: 11q13
Cellular Component: cytoplasm; cytosol; extracellular space; intracellular; mitochondrion; nucleus; plasma membrane; vesicle
Molecular Function: drug binding; glutathione binding; glutathione transferase activity; JUN kinase binding; kinase regulator activity; protein binding
Biological Process: cellular response to insulin stimulus; central nervous system development; glutathione metabolic process; negative regulation of acute inflammatory response; negative regulation of apoptosis; negative regulation of biosynthetic process; negative regulation of fibroblast proliferation; negative regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of interleukin-1 beta production; negative regulation of JNK activity; negative regulation of MAP kinase activity; negative regulation of MAPKKK cascade; negative regulation of nitric-oxide synthase biosynthetic process; negative regulation of protein kinase activity; negative regulation of stress-activated MAPK cascade; negative regulation of tumor necrosis factor production; oligodendrocyte development; organ regeneration; positive regulation of superoxide release; regulation of stress-activated MAPK cascade; response to amino acid stimulus; response to estradiol stimulus; response to ethanol; response to L-ascorbic acid; response to mercury ion; response to reactive oxygen species; response to toxin; xenobiotic metabolic process
Research Articles on GSTP1
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Product Notes
The GSTP1 gstp1 (Catalog #AAA421438) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-GST3/GSTP1 Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's GST3/GSTP1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 32000. Western Blot: Approx 24kDa band observed in Human Kidney, Liver and Lung lysates (calculated MW of 23.4kDa according to NP_000843.1). In transfected HEK293 transiently expressing GSTP1 band of approx.26 kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.01-0.03ug/ml. Researchers should empirically determine the suitability of the GSTP1 gstp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: LADQGQSWKE EV. It is sometimes possible for the material contained within the vial of "GST3/GSTP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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