SDS-Page
NT5M recombinant protein
NT5M, 32-228aa, Human, His tag, E Coli
Gene Names
NT5M; mdN; dNT2; dNT-2
Applications
SDS-Page
Purity
> 95% by SDS-PAGE
Synonyms
NT5M; 32-228aa; Human; His tag; E Coli; 5' (3')-deoxyribonucleotidase mitochondrial; 5' (3')-deoxyribonucleotidase; mitochondrial; DNT2; NT5M recombinant protein
Host
E Coli
Purity/Purification
> 95% by SDS-PAGE
Form/Format
Liquid. 20mM Tris-HCl Buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT
Concentration
0.5 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MGSSHHHHHH SSGLVPRGSH MGGRALRVLV DMDGVLADFE GGFLRKFRAR FPDQPFIALE DRRGFWVSEQ YGRLRPGLSE KAISIWESKN FFFELEPLPG AVEAVKEMAS LQNTDVFICT SPIKMFKYCP YEKYAWVEKY FGPDFLEQIV LTRDKTVVSA DLLIDDRPDI TGAEPTPSWE HVLFTACHNQ HLQLQPPRRR LHSWADDWKA ILDSKRPC
Sequence Length
228
Applicable Applications for NT5M recombinant protein
SDS-PAGE
Antigen Species
Human
Tag Information
His-tag
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
SDS-Page
SDS-Page
Related Product Information for NT5M recombinant protein
NT5M, also known as DNT2, belongs to the 5' (3')-deoxyribonucleotidase family. It localizes to the mitochondrial matrix. This enzyme dephosphorylates the 5'- and 2' (3')-phosphates of uracil and thymine deoxyribonucleotides. The gene is located on chromosome 17 p11.2 in the critical region deleted in the Smith-Magenis syndrome (SMS), a genetic disease of unknown etiology. Recombinant human NT5M protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography.
Product Categories/Family for NT5M recombinant protein
References
Rampazzo C., et al. (2000) Proc. Natl. Acad. Sci. u.S.A. 97:8239-8244; Rinaldo-Matthis A., et al. (2002) Nat. Struct. Biol. 9:779-787
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
25.1 kDa (218aa), confirmed by MALDI-TOF
NCBI Official Full Name
5'(3')-deoxyribonucleotidase, mitochondrial
NCBI Official Synonym Full Names
5',3'-nucleotidase, mitochondrial
NCBI Official Symbol
NT5M
NCBI Official Synonym Symbols
mdN; dNT2; dNT-2
NCBI Protein Information
5'(3')-deoxyribonucleotidase, mitochondrial
UniProt Protein Name
5'(3')-deoxyribonucleotidase, mitochondrial
UniProt Gene Name
NT5M
UniProt Synonym Gene Names
DNT2; 5',3'-nucleotidase, mitochondrial; dNT-2
NCBI Description
This gene encodes a 5' nucleotidase that localizes to the mitochondrial matrix. This enzyme dephosphorylates the 5'- and 2'(3')-phosphates of uracil and thymine deoxyribonucleotides. The gene is located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Jul 2008]
Uniprot Description
Dephosphorylates specifically the 5' and 2'(3')-phosphates of uracil and thymine deoxyribonucleotides, and so protects mitochondrial DNA replication from excess dTTP. Has only marginal activity towards dIMP and dGMP.
Research Articles on NT5M
Similar Products
Product Notes
The NT5M nt5m (Catalog #AAA204694) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag. AAA Biotech's NT5M can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the NT5M nt5m for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MGGRALRVLV DMDGVLADFE GGFLRKFRAR FPDQPFIALE DRRGFWVSEQ YGRLRPGLSE KAISIWESKN FFFELEPLPG AVEAVKEMAS LQNTDVFICT SPIKMFKYCP YEKYAWVEKY FGPDFLEQIV LTRDKTVVSA DLLIDDRPDI TGAEPTPSWE HVLFTACHNQ HLQLQPPRRR LHSWADDWKA ILDSKRPC. It is sometimes possible for the material contained within the vial of "NT5M, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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