Loading...

Skip to main content

Call us on + 1 (800) 604-9114 for more information about our products

Looking for specific datasheet Manual/COA/MSDS?
Request a Manual/COA/MSDS

Interested to get a quote about our products?
Request a Quote

Western Blot (WB) (Western blot analysis of GLIS1 Antibody (N-term) in mouse stomach tissue lysates (35ug/lane). GLIS1 (arrow) was detected using the purified Pab.)

Rabbit anti-Human, mouse GLIS1 Polyclonal Antibody | anti-GLIS1 antibody

GLIS1 Antibody (N-term)

Reactivity
Human, mouse
Applications
Western Blot, ELISA, Immunohistochemistry
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
GLIS1; Polyclonal Antibody; GLIS1 Antibody (N-term); Zinc finger protein GLIS1; GLI-similar 1; GLIS1 HGNC:29525); anti-GLIS1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, mouse
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This GLIS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 133-161 amino acids from the N-terminal region of human GLIS1.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.325 (varies by lot)
Sequence Positions
133-161
Sequence Length
620
Applicable Applications for anti-GLIS1 antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of GLIS1 Antibody (N-term) in mouse stomach tissue lysates (35ug/lane). GLIS1 (arrow) was detected using the purified Pab.)

Western Blot (WB) (Western blot analysis of GLIS1 Antibody (N-term) in mouse stomach tissue lysates (35ug/lane). GLIS1 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human lung carcinoma reacted with GLIS1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human lung carcinoma reacted with GLIS1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Related Product Information for anti-GLIS1 antibody
GLIS1 is a GLI (MIM 165220)-related Kruppel-like zinc finger protein that functions as an activator and repressor of transcription (Kim et al., 2002 [PubMed 12042312]).
References
Kim,Y.S., et.al., Nucleic Acids Res. 31 (19), 5513-5525 (2003)
Nakanishi,G., et.al., J. Invest. Dermatol. 126 (1), 49-60 (2006)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
65976
NCBI Official Full Name
zinc finger protein GLIS1
NCBI Official Synonym Full Names
GLIS family zinc finger 1
NCBI Official Symbol
GLIS1
NCBI Protein Information
zinc finger protein GLIS1
UniProt Protein Name
Zinc finger protein GLIS1
Protein Family
UniProt Gene Name
GLIS1
UniProt Entry Name
GLIS1_HUMAN

NCBI Description

GLIS1 is a GLI (MIM 165220)-related Kruppel-like zinc finger protein that functions as an activator and repressor of transcription (Kim et al., 2002 [PubMed 12042312]).[supplied by OMIM, Mar 2008]

Uniprot Description

GLIS1: Acts as both a repressor and activator of transcription. Binds to the consensus sequence 5'-GACCACCCAC-3'. Belongs to the GLI C2H2-type zinc-finger protein family.

Protein type: C2H2-type zinc finger protein

Chromosomal Location of Human Ortholog: 1p32.3

Cellular Component: nucleus

Molecular Function: metal ion binding

Biological Process: transcription from RNA polymerase II promoter; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter

Research Articles on GLIS1

Similar Products

Product Notes

The GLIS1 glis1 (Catalog #AAA9210438) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 133-161. The GLIS1 Antibody (N-term) reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's GLIS1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC). WB~~1:1000. Researchers should empirically determine the suitability of the GLIS1 glis1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GLIS1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

Item has been added to Shopping Cart

If you are ready to order, navigate to Shopping Cart and get ready to checkout.

Looking for a specific manual?
Request a Manual

Request more Information

Please complete the form below and a representative will contact you as soon as possible.

Request a Manual

Please complete the form below and a representative will contact you as soon as possible.

Request a Quote

Please complete the form below and a representative will contact you as soon as possible.