Western Blot (WB)
(Western Blot detection against Immunogen (37kD).)
Mouse anti-Human NT5M Monoclonal Antibody | anti-NT5M antibody
NT5M (5'(3')-deoxyribonucleotidase, Mitochondrial, 5',3'-nucleotidase, Mitochondrial, Deoxy-5'-nucleotidase 2, dNT-2, DNT2, mdN) (HRP)
Gene Names
NT5M; mdN; dNT2; dNT-2
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
NT5M; Monoclonal Antibody; NT5M (5'(3')-deoxyribonucleotidase; Mitochondrial; 5'; 3'-nucleotidase; Deoxy-5'-nucleotidase 2; dNT-2; DNT2; mdN) (HRP); anti-NT5M antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a, lambda
Clone Number
3E2
Specificity
Recognizes human NT5M.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Sequence Length
1607
Applicable Applications for anti-NT5M antibody
ELISA (EIA), Western Blot (WB)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
Partial recombinant corresponding to aa129-228 from human NT5M (AAH35838) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
CTSPIKMFKYCPYEKYAWVEKYFGPDFLEQIVLTRDKTVVSADLLIDDRPDITGAEPTPSWEHVLFTACHNQHLQLQPPRRRLHSWADDWKAILDSKRPC
Conjugate
HRP
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western Blot detection against Immunogen (37kD).)
Western Blot (WB)
(Western Blot detection against Immunogen (37kD).)
Related Product Information for anti-NT5M antibody
Dephosphorylates specifically the 5' and 2'(3')-phosphates of uracil and thymine deoxyribonucleotides, and so protects mitochondrial DNA replication from excess dTTP. Has only marginal activity towards dIMP and dGMP.
Product Categories/Family for anti-NT5M antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
Homo sapiens 5',3'-nucleotidase, mitochondrial, mRNA
NCBI Official Synonym Full Names
5',3'-nucleotidase, mitochondrial
NCBI Official Symbol
NT5M
NCBI Official Synonym Symbols
mdN; dNT2; dNT-2
NCBI Protein Information
5'(3')-deoxyribonucleotidase, mitochondrial
NCBI Description
This gene encodes a 5' nucleotidase that localizes to the mitochondrial matrix. This enzyme dephosphorylates the 5'- and 2'(3')-phosphates of uracil and thymine deoxyribonucleotides. The gene is located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Jul 2008]
Research Articles on NT5M
Similar Products
Product Notes
The NT5M (Catalog #AAA6153876) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The NT5M (5'(3')-deoxyribonucleotidase, Mitochondrial, 5',3'-nucleotidase, Mitochondrial, Deoxy-5'-nucleotidase 2, dNT-2, DNT2, mdN) (HRP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NT5M can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the NT5M for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NT5M, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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