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Neuron-specific Enolase Active Protein | NSE active protein

Neuron-specific enolase (NSE)

Gene Names
Eno2; NSE; RNEN3
Applications
ELISA
Purity
Ion-exchange chromatography and gel-filtration.
Synonyms
Neuron-specific Enolase; Neuron-specific enolase (NSE); NSE active protein
Ordering
For Research Use Only!
Purity/Purification
Ion-exchange chromatography and gel-filtration.
Form/Format
Solution in 0.1 M Tris-HCl, 5 mM Mg2SO4, 0.25 M KCl, pH 5.1 adjusted with sodium phosphate.
Sequence Length
434
Applicable Applications for NSE active protein
ELISA (EIA)
Application Notes
Tested in ELISA and immunoblotting with anti-NSE antibodies.
Source Note
Post mortal human brain.
Blood sample from the tissue donor was tested and found negative for HBsAg, HCV, HIV-1 and 2 antibodies, and syphilis.
Analysis
Purity > 95 % (SDS-PAGE).
Activity
Specific activity is determined for each lot at 25 degree C in 45 mM imidazole, pH 6.8, 45 mM KCl, 1.33 mM Mg2SO4; 1 mM 2-PG. One unit is defined as the amount of NSE converted 1 mol of 2-phospho-D-glycerate per min at 25 degree C (J.Bidegree Chem. 87, 1587-1594, 1980). Subunit composition is gamma-gamma.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
At -20 degree C. Avoid repeated freezing and thawing.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
Molecular Weight
47,141 Da
NCBI Official Full Name
neuron-specific enolase
NCBI Official Synonym Full Names
enolase 2, gamma, neuronal
NCBI Official Symbol
Eno2
NCBI Official Synonym Symbols
NSE; RNEN3
NCBI Protein Information
gamma-enolase; neural enolase; neuron-specific enolase; 2-phospho-D-glycerate hydro-lyase
UniProt Protein Name
Gamma-enolase
UniProt Gene Name
Eno2
UniProt Synonym Gene Names
Eno-2; NSE
UniProt Entry Name
ENOG_RAT

NCBI Description

catalyzes the interconversion of 2-phosphoglycerate to phosphoenolpyruvate; may play a role in glycolysis [RGD, Feb 2006]

Uniprot Description

ENO2: an enzyme with 2-phospho-D-glycerate hydro-lyase activity. One of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates.

Protein type: Carbohydrate Metabolism - glycolysis and gluconeogenesis; Lyase; EC 4.2.1.11

Cellular Component: extracellular space; neuron projection; photoreceptor inner segment; phosphopyruvate hydratase complex; cell soma; cytoplasm; perikaryon; intracellular; myelin sheath

Molecular Function: protein homodimerization activity; protein heterodimerization activity; magnesium ion binding; phosphopyruvate hydratase activity

Biological Process: response to drug; glycolysis; response to organic cyclic substance; response to estradiol stimulus; gluconeogenesis

Research Articles on NSE

Similar Products

Product Notes

The NSE eno2 (Catalog #AAA664045) is an Active Protein and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Neuron-specific Enolase can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). Tested in ELISA and immunoblotting with anti-NSE antibodies. Researchers should empirically determine the suitability of the NSE eno2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Neuron-specific Enolase, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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