Typical Testing Data/Standard Curve (for reference only)
Rat enolase 2 (gamma, neuronal) ELISA Kit | ENO2 elisa kit
Rat Neuron-specific enolase, NSE ELISA Kit
Gene Names
Eno2; NSE; RNEN3
Reactivity
Rat
Synonyms
enolase 2 (gamma; neuronal); Rat Neuron-specific enolase; NSE ELISA Kit; NSE; 2-phospho-D-glycerate hydrolyase; enolase 2; neural enolase; neuron specific gamma enolase; neurone-specific enolase; ENO2 elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of rat NSE. No significant cross-reactivity or interference between rat NSE and analogues was observed.
Sequence Length
434
Samples
Serum, plasma, tissue homogenates.
Assay Type
Sandwich
Detection Range
0.625 ng/ml-40 ng/ml.
Sensitivity
Typically less than 0.156 ng/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for ENO2 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NSE has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NSE present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NSE is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NSE bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
47,141 Da
NCBI Official Full Name
gamma-enolase
NCBI Official Synonym Full Names
enolase 2, gamma, neuronal
NCBI Official Symbol
Eno2
NCBI Official Synonym Symbols
NSE; RNEN3
NCBI Protein Information
gamma-enolase; neural enolase; neuron-specific enolase; 2-phospho-D-glycerate hydro-lyase
UniProt Protein Name
Gamma-enolase
Protein Family
UniProt Gene Name
Eno2
UniProt Synonym Gene Names
Eno-2; NSE
UniProt Entry Name
ENOG_RAT
NCBI Description
catalyzes the interconversion of 2-phosphoglycerate to phosphoenolpyruvate; may play a role in glycolysis [RGD, Feb 2006]
Uniprot Description
Function: Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival.1 PublicationManual assertion based on experiment in:Ref.6
Catalytic activity: 2-phospho-D-glycerate = phosphoenolpyruvate + H2O.UniRule annotationHAMAP-Rule:MF_00318
Cofactor: Magnesium. Required for catalysis and for stabilizing the dimer.
Research Articles on ENO2
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Product Notes
The Rat ENO2 eno2 (Catalog #AAA703638) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA703638 ELISA Kit recognizes Rat ENO2. It is sometimes possible for the material contained within the vial of "enolase 2 (gamma, neuronal), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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