NSE native protein

NSE protein

Cat. Num. AAA537040

• Gene Names: Eno2; NSE; RNEN3
• Applications: User optimized
• Purity: > 95% by SDS-PAGE; NSE protein was purified using Ion-exchange chromatography and gel-filtration.
• Synonyms: NSE; NSE protein; Neural enolase protein; Enolase 2 gamma neuronal protein; Neuron Specific Enolase protein; Neuron specific enolase protein; Enolase 2 protein; gamma-enolase protein; Enolase2 protein; Neuron specific gamma enolase protein; gamma enolase protein; NSE native protein

• Host: Human
• Purity/Purification: > 95% by SDS-PAGE; NSE protein was purified using Ion-exchange chromatography and gel-filtration.
• Form/Format: Frozen liquid in 100mM Tris-HCl, with 5 mM Mg₂SO₄, and 250mM KCl, pH 5.1 adjusted with sodium phosphate.
• Concentration: 0.81mg/ml (varies by lot)
• Sequence Length: 434

• Applicable Applications for NSE native protein: User optimized
• Source: Post mortem human brain
• Protein Type: Native
• Biological Significance: Neuron-specific enolase (NSE), is an enzyme that in humans is encoded by the ENO2 gene. Gamma-enolase is a phosphopyruvate hydratase. Gamma-enolase is one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. Detection of NSE with antibodies can be used to identify neuronal cells and cells with neuroendocrine differentiation.
• Biohazard Information: Blood sample from the tissue donor was tested and found negative for HBsAg, HCV, HIV-1 and 2 antibodies and syphilis
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Aliquot and store at -20 degree C. Avoid repeated Freeze/Thaw cycles; Shipping: Ships with Dry Ice.

SDS-PAGE

(This is a representative image of one lot of the NSE protein. Appearance of the protein on SDS-PAGE gels will vary from lot to lot.)

Related Product Information for NSE native protein

Purified native Human NSE protein

Product Categories/Family for NSE native protein

Neuroscience; Native Protein

NCBI and Uniprot Product Information

• NCBI GI #: 2465396
• NCBI GeneID: 24334
• UniProt Accession #: P07323
• Molecular Weight: 47,141 Da
• NCBI Official Full Name: neuron-specific enolase
• NCBI Official Synonym Full Names: enolase 2, gamma, neuronal
• NCBI Official Symbol: Eno2
• NCBI Official Synonym Symbols: NSE; RNEN3
• NCBI Protein Information: gamma-enolase
• UniProt Protein Name: Gamma-enolase
• UniProt Gene Name: Eno2
• UniProt Synonym Gene Names: Eno-2; NSE
• UniProt Entry Name: ENOG_RAT

NCBI Description

catalyzes the interconversion of 2-phosphoglycerate to phosphoenolpyruvate; may play a role in glycolysis [RGD, Feb 2006]

Uniprot Description

ENO2: an enzyme with 2-phospho-D-glycerate hydro-lyase activity. One of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates.

Protein type: Lyase; Carbohydrate Metabolism - glycolysis and gluconeogenesis; EC 4.2.1.11

Cellular Component: extracellular space; neuron projection; photoreceptor inner segment; phosphopyruvate hydratase complex; cell soma; cytoplasm; perikaryon; intracellular; myelin sheath

Molecular Function: protein homodimerization activity; protein heterodimerization activity; magnesium ion binding; phosphopyruvate hydratase activity

Biological Process: response to drug; glycolysis; response to organic cyclic substance; response to estradiol stimulus; gluconeogenesis