Western Blot (WB)
(All lanes : Anti-MAP2K2 Antibody (T394) at 1:1000 dilutionLane 1: Hela whole cell lysateLane 2: MOLT-4 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 44 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit anti-Human MAP2K2 Polyclonal Antibody | anti-MAP2K2 antibody
MAP2K2 Antibody (T394)
IF:~~1:10~50
IHC-P:~~1:10~50
Western Blot (WB)
(All lanes : Anti-MAP2K2 Antibody (T394) at 1:1000 dilutionLane 1: Hela whole cell lysateLane 2: MOLT-4 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 44 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(All lanes : Anti-MAP2K2 Antibody (T394) at 1:1000 dilutionLane 1: Hela whole cell lysateLane 2: MOLT-4 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 44 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Immunofluorescence (IF)
(Fluorescent image of U251 cell stained with MAP2K2 Antibody (T394) .U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with MAP2K2 primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C). MAP2K2 immunoreactivity is localized to Cytoplasm significantly.)
Immunofluorescence (IF)
(Fluorescent image of U251 cell stained with MAP2K2 Antibody (T394) .U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with MAP2K2 primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C). MAP2K2 immunoreactivity is localized to Cytoplasm significantly.)
Immunohistochemistry (IHC)-Paraffin
(Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with MAP2K2 Antibody (T394), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)-Paraffin
(Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with MAP2K2 Antibody (T394), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Background: Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases (By similarity).
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is known to play a critical role in mitogen growth factor signal transduction. It phosphorylates and thus activates MAPK1/ERK2 and MAPK2/ERK3. The activation of this kinase itself is dependent on the Ser/Thr phosphorylation by MAP kinase kinase kinases. Mutations in this gene cause cardiofaciocutaneous syndrome (CFC syndrome), a disease characterized by heart defects, mental retardation, and distinctive facial features similar to those found in Noonan syndrome. The inhibition or degradation of this kinase is also found to be involved in the pathogenesis of Yersinia and anthrax. A pseudogene, which is located on chromosome 7, has been identified for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
MEK2: a dual-specificity protein kinase of the STE7 kinase family. Phosphorylated and activated by Raf and Mos kinases. Phosphorylates a Thr and a Tyr residue in a Thr-Glu-Tyr sequence located in the activation loop of ERK2 and ERK3. A component of MAP kinase signal transduction pathways involved in mitogen growth factor signal transduction.
Protein type: EC 2.7.12.2; Protein kinase, STE; Kinase, protein; Protein kinase, dual-specificity (non-receptor); STE group; STE7 family
Chromosomal Location of Human Ortholog: 19p13.3
Cellular Component: cell cortex; cytoplasm; cytosol; early endosome; endoplasmic reticulum; extracellular region; focal adhesion; Golgi apparatus; intercellular junction; internal side of plasma membrane; late endosome; microtubule; mitochondrion; nucleus; perinuclear region of cytoplasm; peroxisomal membrane
Molecular Function: ATP binding; MAP kinase kinase activity; metal ion binding; PDZ domain binding; protein binding; protein complex scaffold; protein serine/threonine kinase activator activity; protein serine/threonine kinase activity; protein serine/threonine/tyrosine kinase activity; protein-tyrosine kinase activity; receptor signaling protein serine/threonine kinase activity
Biological Process: activation of MAPK activity; heart development; MAPKKK cascade; peptidyl-tyrosine phosphorylation; positive regulation of axonogenesis; regulation of axon regeneration; regulation of stress-activated MAPK cascade; thymus development; thyroid gland development
Disease: Cardiofaciocutaneous Syndrome 4
Research Articles on MAP2K2
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Product Notes
The MAP2K2 map2k2 (Catalog #AAA9217160) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MAP2K2 Antibody (T394) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MAP2K2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunofluorescence (IF), ELISA (EIA). WB:~~1:1000 IF:~~1:10~50 IHC-P:~~1:10~50. Researchers should empirically determine the suitability of the MAP2K2 map2k2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MAP2K2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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