Enolase, Neuron Specific (NSE) Recombinant Protein | NSE recombinant protein

Recombinant Enolase, Neuron Specific (NSE)

Cat. Num. AAA2011162

• Gene Names: Eno2; NSE; RNEN3
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Enolase; Neuron Specific (NSE); Recombinant Enolase; NSE recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF- SIQKIWARE ILDSRGNPTV EVDLHTAKGL FRAAVPSGAS TGIYEALELR DGDKQRYLGK GVLKAVDHIN STIAPALISS GLSVVEQEKL DNLMLELDGT ENKSKFGANA ILGVSLAVCK AGAAEKDLPL YRHIAQLAGN SDLILPVPAF NVINGGSHAG NKLAMQEFMI LPVGAESFRD AMRLGAEVYH TLKGVIKDKY GKDATNVGDE GGFAPNILEN SEALELVKEA IDKAGYTEKM VIGMDVAASE FYRDGKYDLD FKSPADPSRC TGDQLGALY QDFVRNYPVV SIEDPFDQDD WAAWSKFTAN VGIQIVGDDL TVTNPKRIER AVEEKACNCL LLKVNQIGSV TEAIQACKLA QENGWGVMVS HRSGETEDTF IADLVVGLCT GQIKTGAPCR SERLAKYNQL MRIEEELGEE ARFAGHNFRN PSVL
• Sequence Length: 434

• Applicable Applications for NSE recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Rattus norvegicus (Rat)
• Expression System: Prokaryotic expression
• Residues: Ser2~Leu434 (Accession # P07323) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 26023949
• NCBI GeneID: 24334
• NCBI Accession #: NP_647541.1
• NCBI GenBank Nucleotide #: NM_139325.3
• UniProt Accession #: P07323
• Molecular Weight: 48.5kDa
• NCBI Official Full Name: gamma-enolase
• NCBI Official Synonym Full Names: enolase 2, gamma, neuronal
• NCBI Official Symbol: Eno2
• NCBI Official Synonym Symbols: NSE; RNEN3
• NCBI Protein Information: gamma-enolase; neural enolase; neuron-specific enolase; 2-phospho-D-glycerate hydro-lyase
• UniProt Protein Name: Gamma-enolase
• UniProt Gene Name: Eno2
• UniProt Synonym Gene Names: Eno-2; NSE
• UniProt Entry Name: ENOG_RAT

NCBI Description

catalyzes the interconversion of 2-phosphoglycerate to phosphoenolpyruvate; may play a role in glycolysis [RGD, Feb 2006]

Uniprot Description

ENO2: an enzyme with 2-phospho-D-glycerate hydro-lyase activity. One of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates.

Protein type: Carbohydrate Metabolism - glycolysis and gluconeogenesis; Lyase; EC 4.2.1.11

Cellular Component: extracellular space; neuron projection; phosphopyruvate hydratase complex; photoreceptor inner segment; cell soma; cytoplasm; perikaryon; intracellular; myelin sheath

Molecular Function: protein homodimerization activity; protein heterodimerization activity; magnesium ion binding; phosphopyruvate hydratase activity

Biological Process: response to drug; glycolysis; response to organic cyclic substance; response to estradiol stimulus; gluconeogenesis