Typical Testing Data/Standard Curve
Rat Neuropilin 2 ELISA Kit | NRP2 elisa kit
Rat Neuropilin 2 ELISA Kit
Reactivity
Rat
Applications
ELISA
Synonyms
Neuropilin 2; Rat Neuropilin 2 ELISA Kit; VEGF165R2; Neuropilin-2; Vascular endothelial cell growth factor 165 receptor 2; NRP2 elisa kit
Reactivity
Rat
Specificity
The Rat Neuropilin 2 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Rat Neuropilin 2 proteins within the range of 156 pg/ml - 10000 pg/ml.
Sequence Length
925
Applicable Applications for NRP2 elisa kit
Sandwich ELISA (EIA)
Samples
Cell Culture Supernatants, Cell Lysates, Tissue Homogenates.
Sensitivity
10 pg/ml.
Preparation and Storage
Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.
Typical Testing Data/Standard Curve
Typical Testing Data/Standard Curve
Related Product Information for NRP2 elisa kit
Principle of the Assay||The Rat Neuropilin 2 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat Neuropilin 2 in Cell Culture Supernatants, Cell Lysates, Tissue Homogenates. This assay employs an antibody specific for Rat Neuropilin 2 coated on a 96-well plate. Standards and samples are pipetted into the wells and Neuropilin 2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat Neuropilin 2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Neuropilin 2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Background/Introduction: NRP2 (Neuropilin 2), also called Npn2 or VEGF165R2, encodes a member of the neuropilin family of receptor proteins. The soluble NRP2 was secreted as a 62.5-kD protein following transfection in Chinese hamster ovary cells. The NRP2 gene is mapped to 2q33.3. The NRP2 gene contains 17 exons and spans about 112 kb. This protein may play a role in cardiovascular development, axon guidance, and tumorigenesis. Mice with null mutations in genes encoding Sema3F, and its holoreceptor components Npn2 and plexin A3 (PLEXA3), exhibit increased dentate gyrus granule cell and cortical layer V pyramidal neuron spine number and size, and also aberrant spine distribution. Moreover, Sema3F promotes loss of spines and excitatory synapses in dissociated neurons in vitro, and in NRP2-null brain slices cortical layer V and dentate gyrus granule cells exhibit increased miniature excitatory postsynaptic current frequency. These disparate effects of secreted semaphorins are reflected in the restricted dendritic localization of NRP2 to apical dendrites and of Npn1 to all dendrites of cortical pyramidal neurons.
Background/Introduction: NRP2 (Neuropilin 2), also called Npn2 or VEGF165R2, encodes a member of the neuropilin family of receptor proteins. The soluble NRP2 was secreted as a 62.5-kD protein following transfection in Chinese hamster ovary cells. The NRP2 gene is mapped to 2q33.3. The NRP2 gene contains 17 exons and spans about 112 kb. This protein may play a role in cardiovascular development, axon guidance, and tumorigenesis. Mice with null mutations in genes encoding Sema3F, and its holoreceptor components Npn2 and plexin A3 (PLEXA3), exhibit increased dentate gyrus granule cell and cortical layer V pyramidal neuron spine number and size, and also aberrant spine distribution. Moreover, Sema3F promotes loss of spines and excitatory synapses in dissociated neurons in vitro, and in NRP2-null brain slices cortical layer V and dentate gyrus granule cells exhibit increased miniature excitatory postsynaptic current frequency. These disparate effects of secreted semaphorins are reflected in the restricted dendritic localization of NRP2 to apical dendrites and of Npn1 to all dendrites of cortical pyramidal neurons.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
103,897 Da
NCBI Official Full Name
Neuropilin-2
NCBI Official Synonym Full Names
neuropilin 2
NCBI Official Symbol
Nrp2
NCBI Protein Information
neuropilin-2
UniProt Protein Name
Neuropilin-2
Protein Family
UniProt Gene Name
Nrp2
UniProt Entry Name
NRP2_RAT
NCBI Description
an axonal guidance molecule [RGD, Feb 2006]
Uniprot Description
High affinity receptor for semaphorins 3C, 3F, VEGF-165 and VEGF-145 isoforms of VEGF, and the PLGF-2 isoform of PGF.
Research Articles on NRP2
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Product Notes
The Rat NRP2 nrp2 (Catalog #AAA8244577) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA8244577 ELISA Kit recognizes Rat NRP2. AAA Biotech's Neuropilin 2 can be used in a range of immunoassay formats including, but not limited to, Sandwich ELISA (EIA). Researchers should empirically determine the suitability of the NRP2 nrp2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Neuropilin 2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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