Western Blot (WB)
(Western blot analysis of neuropilin-2 (NRP-2) expression in neonatal rat brain. The blot was probed with mouse monoclonal anti-NRP-2 (MAM Domain) at 1:250.)
Mouse Neuropilin-2 Monoclonal Antibody | anti-Nrp2 antibody
Neuropilin-2 (MAM Domain) Antibody
Reactivity
Human, Rat, Mouse
Applications
Western Blot, ELISA
Purity
Protein A chromatography
Synonyms
Neuropilin-2; Monoclonal Antibody; Neuropilin-2 (MAM Domain) Antibody; Neuropilin-2 (MAM Domain); anti-Nrp2 antibody
Host
Mouse
Reactivity
Human, Rat, Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
M391
Specificity
This antibody detects a 130kDa* protein corresponding to the apparent molecular mass of neuropilin-2 on SDS-PAGE immunoblots of rat brain
Purity/Purification
Protein A chromatography
Form/Format
Mouse monoclonal antibody purified with protein A chromatography is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.
Applicable Applications for anti-Nrp2 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
ELISA: 1:2000
WB: 1:500
WB: 1:500
Immunogen
Clone M391 was generated from Neuropilin-2 recombinant protein containing amino acids in the MAM domain region of rat neuropilin-2. This sequence is highly conserved in human and mouse neuropilin-2, and has low homology to neuropilin-1.
Preparation and Storage
Store at -20 degree C. Stable for 1 year.
Western Blot (WB)
(Western blot analysis of neuropilin-2 (NRP-2) expression in neonatal rat brain. The blot was probed with mouse monoclonal anti-NRP-2 (MAM Domain) at 1:250.)
Western Blot (WB)
(Western blot analysis of neuropilin-2 (NRP-2) expression in neonatal rat brain. The blot was probed with mouse monoclonal anti-NRP-2 (MAM Domain) at 1:250.)
Related Product Information for anti-Nrp2 antibody
Neuropilins are transmembrane proteins that contain two CUB domains (a1 and a2), two coagulation factor-like domains (b1 and b2), and a MAM domain in the extracellular region. These proteins have short cytoplasmic domains that include a PDZ-binding motif. The neuropilin (NRP) family includes NRP-1, NRP-2A, and NRP-2B. NRP-1 and NRP-2 can form a heterodimer. NRP-1 binds with high affinity to Semaphorin 3A, while NRP-2 binds with high affinity to Semaphorin 3F. Both NRP-1 and NRP-2 have been implicated in VEGFR and Plexin receptor function, and both neuropilins may be involved in axon guidance, angiogenesis, and tumor progression.
References
Nakamura, F. & Goshima, Y. (2002) Adv Exp Med Biol. 515:55.
He, Z. & Tessier-Lavigne, M. (1997) Cell 90:739.
Kolodkin, A.L. et al. (1997) Cell 90:753.
He, Z. & Tessier-Lavigne, M. (1997) Cell 90:739.
Kolodkin, A.L. et al. (1997) Cell 90:753.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
103,897 Da
NCBI Official Full Name
Neuropilin-2
NCBI Official Synonym Full Names
neuropilin 2
NCBI Official Symbol
Nrp2
NCBI Protein Information
neuropilin-2; vascular endothelial cell growth factor 165 receptor 2
UniProt Protein Name
Neuropilin-2
Protein Family
UniProt Gene Name
Nrp2
UniProt Entry Name
NRP2_RAT
NCBI Description
an axonal guidance molecule [RGD, Feb 2006]
Uniprot Description
High affinity receptor for semaphorins 3C, 3F, VEGF-165 and VEGF-145 isoforms of VEGF, and the PLGF-2 isoform of PGF.
Research Articles on Nrp2
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Product Notes
The Nrp2 nrp2 (Catalog #AAA474470) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Neuropilin-2 (MAM Domain) Antibody reacts with Human, Rat, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Neuropilin-2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). ELISA: 1:2000 WB: 1:500. Researchers should empirically determine the suitability of the Nrp2 nrp2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Neuropilin-2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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