Flow Cytometry (FC/FACS)
(Flow Cytometry of chicken PBMC were double stained with MBS631726 TCR, alpha, beta, V beta 1 (T Cell Receptor) (PE) and mouse anti-chicken CD3-FITC. Small|lymphocytes were gated and subjected to 2-color analysis on a FACScan (BDIS, San Jose, CA).)
Mouse anti-Chicken TCR, alpha, beta, V beta 1 Monoclonal Antibody
TCR, alpha, beta, V beta 1 (T Cell Receptor) (PE)
Reactivity
Chicken
Applications
Immunoprecipitation, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
PurifiedPurified
Synonyms
TCR; alpha; beta; V beta 1; Monoclonal Antibody; V beta 1 (T Cell Receptor) (PE); Anti -TCR; anti-TCR; V beta 1 antibody
Host
Mouse
Reactivity
Chicken
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
5K82
Specificity
Recognizes chicken TCR alpha, beta. Reacts with approximately 40% of thymocytes, 40-50% of blood mononuclear cells and 40% of splenocytes in the chicken.
Purity/Purification
Purified
Purified
Purified
Form/Format
Supplied as a liquid in PBS, 0.1% sodium azide and a stabilizing agent. Conjugated to PE.
Applicable Applications for anti-TCR, alpha, beta, V beta 1 antibody
Immunoprecipitation (IP), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
Flow cytometry 1, , Immunohistochemistry (acetone-fixed, frozen sections), , In ovo suppression of the development of TCR-Vb1 cells 5,6, , Immunoprecipitation 1
Dilution: Flow Cytometry: 0.2ug/10e6 cells
Dilution: Flow Cytometry: 0.2ug/10e6 cells
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20 degree C or colder. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Flow Cytometry (FC/FACS)
(Flow Cytometry of chicken PBMC were double stained with MBS631726 TCR, alpha, beta, V beta 1 (T Cell Receptor) (PE) and mouse anti-chicken CD3-FITC. Small|lymphocytes were gated and subjected to 2-color analysis on a FACScan (BDIS, San Jose, CA).)
Flow Cytometry (FC/FACS)
(Flow Cytometry of chicken PBMC were double stained with MBS631726 TCR, alpha, beta, V beta 1 (T Cell Receptor) (PE) and mouse anti-chicken CD3-FITC. Small|lymphocytes were gated and subjected to 2-color analysis on a FACScan (BDIS, San Jose, CA).)
Related Product Information for anti-TCR, alpha, beta, V beta 1 antibody
This antibody precipitates a CD3-associated heterodimer of Mr 90kD (two bands of Mr 40kD and 50kD upon reduction) on chicken peripheral blood T cells.1 Deglycosylation of the heterodimer yields two polypeptides of Mr 34kD and 29kD from antibody precipitates. In the chicken, two distinct subpopulations of alpha/beta T cells appear in the thymus subsequent to the appearance of gamma/delta T cells. These subpopulations, originally denoted as TCR2 and TCR3,1-3 arise sequentially in the thymus during ontogeny and are now known to represent two distinct Vb families, Vb1 and Vb2, respectively.4 Two-color immunofluorescence has revealed that most of the TCR2+ thymocytes express both CD4 and CD8 antigens. The TCR2+ cells in blood were found to express either CD4 (74±2%) or CD8 (26±4%). TCR2+ cells in the spleen also express either CD4 (37±1%) or CD8 (64±4%). Surprisingly, a relatively large subpopulation of CD8+ cells in the spleen are negative for TCR2.1-3 This observation led to the demonstration that 71±6% of the TCR1(gamma/delta)+ cells in the spleen express the CD8 marker.1-4
Product Categories/Family for anti-TCR, alpha, beta, V beta 1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
TCR beta chain
Similar Products
Product Notes
The TCR, alpha, beta, V beta 1 (Catalog #AAA631726) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The TCR, alpha, beta, V beta 1 (T Cell Receptor) (PE) reacts with Chicken and may cross-react with other species as described in the data sheet. AAA Biotech's TCR, alpha, beta, V beta 1 can be used in a range of immunoassay formats including, but not limited to, Immunoprecipitation (IP), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). Flow cytometry 1,, Immunohistochemistry (acetone-fixed, frozen sections),, In ovo suppression of the development of TCR-Vb1 cells 5,6,, Immunoprecipitation 1 Dilution: Flow Cytometry: 0.2ug/10e6 cells. Researchers should empirically determine the suitability of the TCR, alpha, beta, V beta 1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TCR, alpha, beta, V beta 1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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