Typical Testing Data/Standard Curve (for reference only)
(Rat Neuropilin-2 PicoKine ELISA Kit standard curve )
Rat Neuropilin-2 ELISA Kit | NRP2 elisa kit
Rat Neuropilin-2 PicoKine ELISA Kit
Reactivity
Rat
Synonyms
Neuropilin-2; Rat Neuropilin-2 PicoKine ELISA Kit; neuropilin 2; MGC126574; Neuropilin 2; Neuropilin2; NP 2; NP2; NPN 2; NPN2; NRP 2; Nrp2; NRP2_HUMAN; PRO2714; Receptor for VEGF165 and semaphorins class3; Vascular endothelial cell growth factor 165 receptor 2; Vascular endothelial growth factor 165 receptor 2; VEGF1265R2; VEGF165R2; NRP2 elisa kit
Reactivity
Rat
Specificity
Natural and recombinant Rat Neuropilin-2
Sequence Length
925
Samples
Cell culture supernates, cell lysates and tissue homogenates.
Assay Type
Sandwich
Detection Range
156pg/ml-10000pg/ml
Sensitivity
<10pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Typical Testing Data/Standard Curve (for reference only)
(Rat Neuropilin-2 PicoKine ELISA Kit standard curve )
Typical Testing Data/Standard Curve (for reference only)
(Rat Neuropilin-2 PicoKine ELISA Kit standard curve )
Related Product Information for NRP2 elisa kit
Principle of the Assay: Rat Neuropilin-2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Neuropilin-2 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO; Immunogen sequence: Q23-D857) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Neuropilin-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat Neuropilin-2 amount of sample captured in plate.
Background: NRP2(Neuropilin-2), also called Npn2 or VEGF165R2, encodes a member of the neuropilin family of receptor proteins. The soluble NRP2 was secreted as a 62.5-kD protein following transfection in Chinese hamster ovary cells. The NRP2 gene is mapped to 2q33.3. The NRP2 gene contains 17 exons and spans about 112 kb. This protein may play a role in cardiovascular development, axon guidance, and tumorigenesis. Mice with null mutations in genes encoding Sema3F, and its holoreceptor components Npn2 and plexin A3 (PLEXA3), exhibit increased dentate gyrus granule cell and cortical layer V pyramidal neuron spine number and size, and also aberrant spine distribution. Moreover, Sema3F promotes loss of spines and excitatory synapses in dissociated neurons in vitro, and in NRP2-null brain slices cortical layer V and dentate gyrus granule cells exhibit increased miniature excitatory postsynaptic current frequency. These disparate effects of secreted semaphorins are reflected in the restricted dendritic localization of NRP2 to apical dendrites and of Npn1 to all dendrites of cortical pyramidal neurons.
Background: NRP2(Neuropilin-2), also called Npn2 or VEGF165R2, encodes a member of the neuropilin family of receptor proteins. The soluble NRP2 was secreted as a 62.5-kD protein following transfection in Chinese hamster ovary cells. The NRP2 gene is mapped to 2q33.3. The NRP2 gene contains 17 exons and spans about 112 kb. This protein may play a role in cardiovascular development, axon guidance, and tumorigenesis. Mice with null mutations in genes encoding Sema3F, and its holoreceptor components Npn2 and plexin A3 (PLEXA3), exhibit increased dentate gyrus granule cell and cortical layer V pyramidal neuron spine number and size, and also aberrant spine distribution. Moreover, Sema3F promotes loss of spines and excitatory synapses in dissociated neurons in vitro, and in NRP2-null brain slices cortical layer V and dentate gyrus granule cells exhibit increased miniature excitatory postsynaptic current frequency. These disparate effects of secreted semaphorins are reflected in the restricted dendritic localization of NRP2 to apical dendrites and of Npn1 to all dendrites of cortical pyramidal neurons.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
103,897 Da
NCBI Official Full Name
Neuropilin-2
NCBI Official Synonym Full Names
neuropilin 2
NCBI Official Symbol
Nrp2
NCBI Protein Information
neuropilin-2; vascular endothelial cell growth factor 165 receptor 2
UniProt Protein Name
Neuropilin-2
Protein Family
UniProt Gene Name
Nrp2
UniProt Entry Name
NRP2_RAT
NCBI Description
an axonal guidance molecule [RGD, Feb 2006]
Uniprot Description
High affinity receptor for semaphorins 3C, 3F, VEGF-165 and VEGF-145 isoforms of VEGF, and the PLGF-2 isoform of PGF.
Research Articles on NRP2
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Product Notes
The Rat NRP2 nrp2 (Catalog #AAA177235) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA177235 ELISA Kit recognizes Rat NRP2. It is sometimes possible for the material contained within the vial of "Neuropilin-2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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