Human nuclear receptor subfamily 4, group A, member 2 ELISA Kit | NR4A2 elisa kit

Human Nuclear receptor subfamily 4 group A member 2, NR4A2 ELISA Kit

Cat. Num. AAA912795

• Gene Names: NR4A2; NOT; RNR1; HZF-3; NURR1; TINUR
• Reactivity: Human
• Synonyms: nuclear receptor subfamily 4; group A; member 2; Human Nuclear receptor subfamily 4 group A member 2; NR4A2 ELISA Kit; Human Nuclear receptor subfamily 4 group A member 2 (NR4A2) ELISA kit; HZF-3; NOT; NURR1; RNR1; TINUR; NGFI-B/nur77 beta-type transcription factor homolog; OTTHUMP00000204707; T-cell nuclear receptor NOT; intermediate-early receptor protein; nur related protein-1; human ho; NR4A2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human NR4A2. No significant cross-reactivity or interference between human NR4A2 and analogues was observed.
• Sequence Length: 598

• Samples: Serum, plasma, tissue homogenates, cell lysates.
• Assay Type: Sandwich
• Detection Range: 31.25 pg/ml -2000 pg/ml.
• Sensitivity: The minimum detectable dose of human NR4A2 is typically less than 7.81 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NR4A2 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NR4A2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NR4A2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NR4A2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NR4A2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 5453822
• NCBI GeneID: 4929
• NCBI Accession #: NP_006177.1
• NCBI GenBank Nucleotide #: NM_006186.3
• UniProt Accession #: P43354; Q16311; Q53RZ2
• Molecular Weight: 66,591 Da
• NCBI Official Full Name: nuclear receptor subfamily 4 group A member 2
• NCBI Official Synonym Full Names: nuclear receptor subfamily 4, group A, member 2
• NCBI Official Symbol: NR4A2
• NCBI Official Synonym Symbols: NOT; RNR1; HZF-3; NURR1; TINUR
• NCBI Protein Information: nuclear receptor subfamily 4 group A member 2; nuclear receptor related 1; T-cell nuclear receptor NOT; orphan nuclear receptor NR4A2; orphan nuclear receptor NURR1; intermediate-early receptor protein; immediate-early response protein NOT; nur related protein-1, human homolog of; transcriptionally-inducible nuclear receptor; NGFI-B/nur77 beta-type transcription factor homolog; transcriptionally inducible nuclear receptor related 1
• UniProt Protein Name: Nuclear receptor subfamily 4 group A member 2
• Protein Family: Nuclear receptor subfamily
• UniProt Gene Name: NR4A2
• UniProt Synonym Gene Names: NOT; NURR1; TINUR
• UniProt Entry Name: NR4A2_HUMAN

NCBI Description

This gene encodes a member of the steroid-thyroid hormone-retinoid receptor superfamily. The encoded protein may act as a transcription factor. Mutations in this gene have been associated with disorders related to dopaminergic dysfunction, including Parkinson disease, schizophernia, and manic depression. Misregulation of this gene may be associated with rheumatoid arthritis. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

NR4A2: Transcriptional regulator which is important for the differentiation and maintenance of meso-diencephalic dopaminergic (mdDA) neurons during development. It is crucial for expression of a set of genes such as SLC6A3, SLC18A2, TH and DRD2 which are essential for development of mdDA neurons. Belongs to the nuclear hormone receptor family. NR4 subfamily.

Protein type: Nuclear receptor; DNA-binding

Chromosomal Location of Human Ortholog: 2q22-q23

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: protein binding; ligand-dependent nuclear receptor activity; zinc ion binding; protein heterodimerization activity; steroid hormone receptor activity

Biological Process: positive regulation of catalytic activity; transcription initiation from RNA polymerase II promoter; response to inorganic substance; intracellular receptor-mediated signaling pathway; transcription, DNA-dependent; adult locomotory behavior; death; regulation of dopamine metabolic process; response to amphetamine; neuron migration; central nervous system projection neuron axonogenesis; dopamine biosynthetic process; response to insecticide; signal transduction; neuron maturation; post-embryonic development; habenula development; regulation of respiratory gaseous exchange; general adaptation syndrome; cellular response to extracellular stimulus; response to hypoxia; positive regulation of transcription from RNA polymerase II promoter; steroid hormone mediated signaling; gene expression; negative regulation of neuron apoptosis

Disease: Parkinson Disease, Late-onset