Western Blot (WB)
(Cell lysates of LNcap (30ug) and Rat testis (30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human STEAP1 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
Mouse anti-Human, Rat STEAP1 Monoclonal Antibody | anti-STEAP1 antibody
STEAP1 antibody
Gene Names
STEAP1; STEAP; PRSS24
Reactivity
Human, Rat
Applications
ELISA, Western Blot, Immunofluorescence, Immunohistochemistry
Purity
By protein-G affinity chromatography
Synonyms
STEAP1; Monoclonal Antibody; STEAP1 antibody; six transmembrane epithelial antigen of the prostate 1; six transmembrane epithelial antigen of the prostate; PRSS24; anti-STEAP1 antibody
Host
Mouse
Reactivity
Human, Rat
Clonality
Monoclonal
Isotype
IgG2b, k
Clone Number
J2D2
Purity/Purification
By protein-G affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
339
Applicable Applications for anti-STEAP1 antibody
ELISA (EIA), Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)
Application Notes
The antibody has been tested by ELISA, Western blot, immunofluorescence and immunohistochemistry analysis to assure specificity and reactivity.
Recommended dilution range for Western blot is 1:1,000~1:2,000, immunofluorescence 1:100 ~1:300 and immunohistochemistry analysis is 1:50~100.
Recommended starting dilution for Western blot is 1:1,000, Immunofluorescence 1:100 and Immunohistochemistry analysis is 1:50.
Recommended dilution range for Western blot is 1:1,000~1:2,000, immunofluorescence 1:100 ~1:300 and immunohistochemistry analysis is 1:50~100.
Recommended starting dilution for Western blot is 1:1,000, Immunofluorescence 1:100 and Immunohistochemistry analysis is 1:50.
Antigen Species
Human
Immunogen
Recombinant human STEAP1 (1-70aa) purified from E. coli
Clone Note
Anti-human STEAP1 mAb, clone J2D2, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human STEAP1 protein.
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Western Blot (WB)
(Cell lysates of LNcap (30ug) and Rat testis (30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human STEAP1 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
Western Blot (WB)
(Cell lysates of LNcap (30ug) and Rat testis (30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human STEAP1 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
Immunohistochemistry (IHC)
(Paraffin embedded sections of human breast cancer and prostate hyperplasia tissue were incubated with anti-human STEAP1 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB))
Immunohistochemistry (IHC)
(Paraffin embedded sections of human breast cancer and prostate hyperplasia tissue were incubated with anti-human STEAP1 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB))
Immunofluorescence (IF)
(Immunofluorescence staining of human LNCap cell colony with monoclonal anti- human STEAP1 antibody (J2D2) )
Immunofluorescence (IF)
(Immunofluorescence staining of human LNCap cell colony with monoclonal anti- human STEAP1 antibody (J2D2) )
Related Product Information for anti-STEAP1 antibody
Six-transmembrane epithelial antigen of the prostate (STEAP) is expressed predominantly in human prostate tissue and in other common malignancies including prostate, bladder, colon, and ovarian carcinomas, and in Ewing's sarcoma, suggesting that it could function as an almost universal tumor antigen. Immunohistochemical analysis indicated STEAP-1 localization at cell-cell junctions of the secretory epithelium of prostate and prostate cancer cells. The favorable expression profiling of STEAP-1 in normal and cancer tissues suggested its potential use as a target for immunotherapy.
Product Categories/Family for anti-STEAP1 antibody
References
David A. Rodeberg, et al. (2005) Clin Cancer Res. June; 11 (12): 4545-4552.; Pia M. Challita-Eid, et al., (2007) Cancer research June 67 (12): 5798-5805.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
39,851 Da
NCBI Official Full Name
metalloreductase STEAP1
NCBI Official Synonym Full Names
STEAP family member 1
NCBI Official Symbol
STEAP1
NCBI Official Synonym Symbols
STEAP; PRSS24
NCBI Protein Information
metalloreductase STEAP1
UniProt Protein Name
Metalloreductase STEAP1
Protein Family
UniProt Gene Name
STEAP1
UniProt Synonym Gene Names
PRSS24; STEAP
NCBI Description
This gene is predominantly expressed in prostate tissue, and is found to be upregulated in multiple cancer cell lines. The gene product is predicted to be a six-transmembrane protein, and was shown to be a cell surface antigen significantly expressed at cell-cell junctions. [provided by RefSeq, Jul 2008]
Uniprot Description
Metalloreductase that has the ability to reduce both Fe3+ to Fe2+ and Cu2+ to Cu1+. Uses NAD+ as acceptor ().
Research Articles on STEAP1
Similar Products
Product Notes
The STEAP1 steap1 (Catalog #AAA200111) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The STEAP1 antibody reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's STEAP1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC). The antibody has been tested by ELISA, Western blot, immunofluorescence and immunohistochemistry analysis to assure specificity and reactivity. Recommended dilution range for Western blot is 1:1,000~1:2,000, immunofluorescence 1:100 ~1:300 and immunohistochemistry analysis is 1:50~100. Recommended starting dilution for Western blot is 1:1,000, Immunofluorescence 1:100 and Immunohistochemistry analysis is 1:50. Researchers should empirically determine the suitability of the STEAP1 steap1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "STEAP1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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