Human Neuropeptide Y Receptor Y2 ELISA Kit | NPYR2 elisa kit

Human Neuropeptide Y Receptor Y2 ELISA Kit

Cat. Num. AAA762919

• Gene Names: NPY2R; NPY2-R
• Reactivity: Human
• Synonyms: Neuropeptide Y Receptor Y2; Human Neuropeptide Y Receptor Y2 ELISA Kit; NPYR2; NPYR2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of NPYR2. No significant cross-reactivity or interference between NPYR2 and analogues was observed.
• Sequence Length: 381

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.313-20ng/ml
• Sensitivity: 0.188ng/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NPYR2 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 29824918
• NCBI GeneID: 4887
• Molecular Weight: 42,731 Da
• NCBI Official Full Name: neuropeptide Y receptor Y2
• NCBI Official Synonym Full Names: neuropeptide Y receptor Y2
• NCBI Official Symbol: NPY2R
• NCBI Official Synonym Symbols: NPY2-R
• NCBI Protein Information: neuropeptide Y receptor type 2
• UniProt Protein Name: Neuropeptide Y receptor type 2
• UniProt Gene Name: NPY2R
• UniProt Synonym Gene Names: NPY2-R; Y2 receptor
• UniProt Entry Name: NPY2R_HUMAN

Uniprot Description

NPY2R: Receptor for neuropeptide Y and peptide YY. The rank order of affinity of this receptor for pancreatic polypeptides is PYY > NPY > PYY (3-36) > NPY (2-36) > [Ile-31, Gln-34] PP > [Leu- 31, Pro-34] NPY > PP, [Pro-34] PYY and NPY free acid. Belongs to the G-protein coupled receptor 1 family.

Protein type: Membrane protein, multi-pass; Receptor, GPCR; Membrane protein, integral; GPCR, family 1

Chromosomal Location of Human Ortholog: 4q31

Cellular Component: cytosol; integral to plasma membrane; nonmotile primary cilium; plasma membrane

Molecular Function: calcium channel regulator activity; neuropeptide Y receptor activity; peptide YY receptor activity; protein binding; receptor activity

Biological Process: behavioral fear response; cell surface receptor linked signal transduction; elevation of cytosolic calcium ion concentration; feeding behavior; G-protein signaling, adenylate cyclase inhibiting pathway; locomotory behavior; negative regulation of cAMP biosynthetic process; negative regulation of neurological process; negative regulation of secretion; negative regulation of synaptic transmission, glutamatergic; neuropeptide signaling pathway; nitric oxide mediated signal transduction; positive regulation of circadian sleep/wake cycle, non-REM sleep; positive regulation of dopamine secretion; positive regulation of peptide secretion; positive regulation of smooth muscle contraction; regulation of sensory perception of pain; secretion