AKR1C2 recombinant protein

AKR1C2 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121635

• Gene Names: AKR1C2; DD; DD2; BABP; DDH2; HBAB; HAKRD; MCDR2; SRXY8; AKR1C-pseudo
• Purity: >90% as determined by SDS-PAGE
• Synonyms: AKR1C2; AKR1C2 Protein; Human; Recombinant (His Tag); Human AKR1C2 Protein (His Tag); AKR1C-pseudo Protein; BABP Protein; DD Protein; DD-2 Protein; DD/BABP Protein; DD2 Protein; DDH2 Protein; HAKRD Protein; HBAB Protein; MCDR2 Protein; SRXY8 Protein; TDD Protein; aldo-keto reductase family 1; member C2; AKR1C2 recombinant protein

• Host: E Coli
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Tyr323

• Species: Human
• Predicted N Terminal: His
• Tag: N-His
• Protein Construction: A DNA sequence encoding the mature form of human AKR1C2 (P52895-1)(Met1-Tyr323) was expressed with a polyhistidine tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for AKR1C2 recombinant protein

Background: AKR1C2 is a member of the aldo/keto reductase superfamily, which consists of more than 4 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols using NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme binds bile acid with high affinity, and shows minimal 3-alpha-hydroxysteroid dehydrogenase activity. AKR1C2 gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 1p15-p14. Three transcript variants encoding two different isoforms have been found for AKR1C2 gene.

References

Jin Y. et al., 2011, Biochem J. 437 (1): 53-61. Veilleux A. et al., 2012, Am J Physiol Endocrinol Metab. 302 (8): E941-9. Kuang P. et al., 2012, Lung Cancer. 77 (2): 427-32.

NCBI and Uniprot Product Information

• NCBI GI #: 207028673
• NCBI GeneID: 1646
• NCBI Accession #: NP_001128713.1
• NCBI GenBank Nucleotide #: NM_001135241.2
• UniProt Accession #: P52895; Q14133; Q5SR16; Q7M4N1; Q96A71; A8K2N9; B4DKR9
• Molecular Weight: 36,735 Da
• NCBI Official Full Name: aldo-keto reductase family 1 member C2 isoform 2
• NCBI Official Synonym Full Names: aldo-keto reductase family 1, member C2
• NCBI Official Symbol: AKR1C2
• NCBI Official Synonym Symbols: DD; DD2; BABP; DDH2; HBAB; HAKRD; MCDR2; SRXY8; AKR1C-pseudo
• NCBI Protein Information: aldo-keto reductase family 1 member C2; DD-2; DD/BABP; 3-alpha-HSD3; pseudo-chlordecone reductase; type II dihydrodiol dehydrogenase; chlordecone reductase homolog HAKRD; trans-1,2-dihydrobenzene-1,2-diol dehydrogenase; dihydrodiol dehydrogenase 2; bile a
• UniProt Protein Name: Aldo-keto reductase family 1 member C2
• Protein Family: Aldo-keto reductase family
• UniProt Gene Name: AKR1C2
• UniProt Synonym Gene Names: DDH2; DD-2; DD2; DD/BABP
• UniProt Entry Name: AK1C2_HUMAN

NCBI Description

This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols using NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme binds bile acid with high affinity, and shows minimal 3-alpha-hydroxysteroid dehydrogenase activity. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]

Uniprot Description

AKR1C2: Works in concert with the 5-alpha/5-beta-steroid reductases to convert steroid hormones into the 3-alpha/5-alpha and 3-alpha/5-beta-tetrahydrosteroids. Catalyzes the inactivation of the most potent androgen 5-alpha-dihydrotestosterone (5-alpha- DHT) to 5-alpha-androstane-3-alpha,17-beta-diol (3-alpha-diol). Has a high bile-binding ability. Defects in AKR1C2 are a cause of 46,XY sex reversal type 8 (SRXY8). A disorder of sex development. Affected individuals have a 46,XY karyotype but present as phenotypically normal females. Belongs to the aldo/keto reductase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 1.1.1.357; Oxidoreductase; Xenobiotic Metabolism - metabolism by cytochrome P450; EC 1.3.1.20

Chromosomal Location of Human Ortholog: 10p15-p14

Cellular Component: cytoplasm

Molecular Function: oxidoreductase activity, acting on NADH or NADPH, quinone or similar compound as acceptor; ketosteroid monooxygenase activity; carboxylic acid binding; bile acid binding; trans-1,2-dihydrobenzene-1,2-diol dehydrogenase activity; aldehyde reductase activity; phenanthrene 9,10-monooxygenase activity

Biological Process: steroid metabolic process; positive regulation of protein kinase B signaling cascade; G-protein coupled receptor protein signaling pathway; epithelial cell differentiation; digestion; positive regulation of cell proliferation; progesterone metabolic process; prostaglandin metabolic process

Disease: 46,xy Sex Reversal 8