Rat Neuropeptide Y Receptor Y2 (NPY2R) ELISA Kit | NPY2R elisa kit

Rat Neuropeptide Y Receptor Y2 (NPY2R) ELISA Kit

Cat. Num. AAA2021003

• Gene Names: NPY2R; NPY2-R
• Reactivity: Rat
• Synonyms: Neuropeptide Y Receptor Y2 (NPY2R); Rat Neuropeptide Y Receptor Y2 (NPY2R) ELISA Kit; NPY-Y2 receptor; NPY2R elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Neuropeptide Y Receptor Y2 (NPY2R).; No significant cross-reactivity or interference between Neuropeptide Y Receptor Y2 (NPY2R) and analogues was observed.
• Sequence Length: 381

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.054ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Neuropeptide Y Receptor Y2 (NPY2R) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Neuropeptide Y Receptor Y2 (NPY2R) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NPY2R elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neuropeptide Y Receptor Y2 (NPY2R). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Neuropeptide Y Receptor Y2 (NPY2R). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Neuropeptide Y Receptor Y2 (NPY2R), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Neuropeptide Y Receptor Y2 (NPY2R) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for NPY2R elisa kit

CD & Adhesion molecule

NCBI and Uniprot Product Information

• NCBI GI #: 29824918
• NCBI GeneID: 4887
• Molecular Weight: 42,731 Da
• NCBI Official Full Name: neuropeptide Y receptor Y2
• NCBI Official Synonym Full Names: neuropeptide Y receptor Y2
• NCBI Official Symbol: NPY2R
• NCBI Official Synonym Symbols: NPY2-R
• NCBI Protein Information: neuropeptide Y receptor type 2
• UniProt Protein Name: Neuropeptide Y receptor type 2
• Protein Family: Neuropeptide Y receptor
• UniProt Gene Name: NPY2R
• UniProt Synonym Gene Names: NPY2-R; Y2 receptor
• UniProt Entry Name: NPY2R_HUMAN

Uniprot Description

NPY2R: Receptor for neuropeptide Y and peptide YY. The rank order of affinity of this receptor for pancreatic polypeptides is PYY > NPY > PYY (3-36) > NPY (2-36) > [Ile-31, Gln-34] PP > [Leu- 31, Pro-34] NPY > PP, [Pro-34] PYY and NPY free acid. Belongs to the G-protein coupled receptor 1 family.

Protein type: GPCR, family 1; Membrane protein, multi-pass; Membrane protein, integral; Receptor, GPCR

Chromosomal Location of Human Ortholog: 4q31

Cellular Component: nonmotile primary cilium; integral to plasma membrane; plasma membrane

Molecular Function: peptide YY receptor activity; protein binding; calcium channel regulator activity; neuropeptide Y receptor activity; receptor activity

Biological Process: positive regulation of dopamine secretion; secretion; positive regulation of cell adhesion; behavioral fear response; negative regulation of synaptic transmission, glutamatergic; positive regulation of peptide secretion; locomotory behavior; elevation of cytosolic calcium ion concentration; negative regulation of cAMP biosynthetic process; neuropeptide signaling pathway; negative regulation of secretion; positive regulation of smooth muscle contraction; G-protein signaling, adenylate cyclase inhibiting pathway; regulation of sensory perception of pain; nitric oxide mediated signal transduction; negative regulation of neurological process; positive regulation of circadian sleep/wake cycle, non-REM sleep