Rat Nitric oxide synthase, endothelial ELISA Kit | Nos3 elisa kit

Rat Nitric oxide synthase, endothelial ELISA Kit

Cat. Num. AAA2881398

• Gene Names: Nos3; eNos
• Reactivity: Rat
• Synonyms: Nitric oxide synthase; endothelial; Rat Nitric oxide synthase; endothelial ELISA Kit; Constitutive NOS; cNOS; EC-NOS; Endothelial NOS; eNOS; NOS type III; NOSIII; Nos3; 1.14.13.39; Nos3 elisa kit

• Reactivity: Rat
• Sequence Length: 1202

• Samples: Serum, plasma, tissue homogenates, cell culture supernates or other biological fluids
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Nos3 elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 46402488
• NCBI GeneID: 24600
• NCBI Accession #: NP_068610.1
• NCBI GenBank Nucleotide #: NM_021838.2
• UniProt Accession #: Q62600; O88672; O89041; Q62734; Q68GV6; Q75NE4
• Molecular Weight: 133,290 Da
• NCBI Official Full Name: nitric oxide synthase, endothelial
• NCBI Official Synonym Full Names: nitric oxide synthase 3
• NCBI Official Symbol: Nos3
• NCBI Official Synonym Symbols: eNos
• NCBI Protein Information: nitric oxide synthase, endothelial
• UniProt Protein Name: Nitric oxide synthase, endothelial
• Protein Family: Nitric oxide synthase
• UniProt Gene Name: Nos3
• UniProt Synonym Gene Names: cNOS; eNOS; NOSIII
• UniProt Entry Name: NOS3_RAT

NCBI Description

enzyme that synthesizes Nitric oxide from L-arginine [RGD, Feb 2006]

Uniprot Description

eNOS: endothelial constitutive nitric oxide synthase. Synthesizes nitric oxide (NO) from arginine and oxygen, which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. Induced in humans by caloric restriction. Stimulated by calcium/calmodulin. Inhibited by NOSIP and NOSTRIN.

Protein type: EC 1.14.13.39; Amino Acid Metabolism - arginine and proline; Oxidoreductase

Cellular Component: apical part of cell; caveola; cytoplasm; cytoskeleton; cytosol; Golgi apparatus; lipid raft; mitochondrion; nucleolus; nucleus; plasma membrane; sarcolemma; vesicle membrane

Molecular Function: actin binding; actin monomer binding; beta-catenin binding; cadherin binding; calmodulin binding; FAD binding; FMN binding; heme binding; Hsp90 protein binding; iron ion binding; NADP binding; nitric-oxide synthase activity; nitric-oxide synthase binding; protein binding

Biological Process: aging; angiogenesis; arginine catabolic process; blood vessel remodeling; endothelial cell migration; female pregnancy; in utero embryonic development; lipopolysaccharide-mediated signaling pathway; lung development; negative regulation of blood pressure; negative regulation of calcium ion transport; negative regulation of cell proliferation; negative regulation of hydrolase activity; negative regulation of muscle hyperplasia; negative regulation of potassium ion transport; negative regulation of smooth muscle cell proliferation; nitric oxide biosynthetic process; nitric oxide mediated signal transduction; ovulation from ovarian follicle; positive regulation of angiogenesis; positive regulation of apoptosis; positive regulation of guanylate cyclase activity; positive regulation of vasodilation; regulation of blood vessel size; regulation of sodium ion transport; regulation of systemic arterial blood pressure by endothelin; regulation of the force of heart contraction by chemical signal; removal of superoxide radicals; response to activity; response to amino acid stimulus; response to axon injury; response to corticosterone stimulus; response to cytokine stimulus; response to drug; response to estradiol stimulus; response to ethanol; response to fructose stimulus; response to hyperoxia; response to hypoxia; response to lead ion; response to lipopolysaccharide; response to lipoprotein stimulus; response to mechanical stimulus; response to metal ion; response to organic cyclic substance; response to peptide hormone stimulus; retina development in camera-type eye; signal transduction; smooth muscle hyperplasia