Western Blot (WB)
(Western Blot: Sample: Rat Serum.)
Rabbit anti-Rat Nitric Oxide Synthase 3, Endothelial (NOS3) Polyclonal Antibody | anti-NOS3 antibody
Polyclonal Antibody to Nitric Oxide Synthase 3, Endothelial (NOS3)
MGHHHHHHSGSEF- AT ILYGSETGRA QSYAQQLGRL FRKAFDPRVL CMDEYDVVSL EHEALVLVVT STFGNGDPPE NGESFAAALM EMSGPYNSSP RPEQHKSYKI RFNSVSCSDP LVSSWRRKRK ESSNTDSAGA LGTLRFCVFG LGSRAYPHFC AFARAVDTRL EELGGERLLQ LGQGDELCGQ EEAFRGWAQA AF
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Western Blot (WB)
(Western Blot: Sample: Rat Serum.)
Western Blot (WB)
(Western Blot: Sample: Rat Serum.)
Western Blot (WB)
(Western Blot: Sample: Recombinant NOS3, Rat.)
Western Blot (WB)
(Western Blot: Sample: Recombinant NOS3, Rat.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P. Samples: Rat Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P. Samples: Rat Tissue))
NCBI and Uniprot Product Information
NCBI Description
enzyme that synthesizes Nitric oxide from L-arginine [RGD, Feb 2006]
Uniprot Description
eNOS: endothelial constitutive nitric oxide synthase. Synthesizes nitric oxide (NO) from arginine and oxygen, which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. Induced in humans by caloric restriction. Stimulated by calcium/calmodulin. Inhibited by NOSIP and NOSTRIN.
Protein type: Amino Acid Metabolism - arginine and proline; EC 1.14.13.39; Oxidoreductase
Chromosomal Location of Human Ortholog: 4q11
Cellular Component: apical part of cell; caveola; cytoplasm; cytoskeleton; cytosol; Golgi apparatus; membrane raft; mitochondrion; nucleolus; nucleus; plasma membrane; sarcolemma; vesicle membrane
Molecular Function: actin binding; actin monomer binding; arginine binding; beta-catenin binding; cadherin binding; calmodulin binding; FAD binding; FMN binding; heme binding; Hsp90 protein binding; iron ion binding; NADP binding; NADPH-hemoprotein reductase activity; nitric-oxide synthase activity; nitric-oxide synthase binding; protein binding; tetrahydrobiopterin binding
Biological Process: aging; angiogenesis; arginine catabolic process; blood vessel remodeling; cellular response to cAMP; cellular response to growth factor stimulus; cellular response to heat; cellular response to magnesium ion; cellular response to organic cyclic compound; cellular response to transforming growth factor beta stimulus; cellular response to tumor necrosis factor; endothelial cell migration; female pregnancy; in utero embryonic development; lipopolysaccharide-mediated signaling pathway; lung development; negative regulation of blood pressure; negative regulation of calcium ion transport; negative regulation of cell proliferation; negative regulation of hydrolase activity; negative regulation of muscle hyperplasia; negative regulation of potassium ion transport; negative regulation of smooth muscle cell proliferation; nitric oxide biosynthetic process; nitric oxide mediated signal transduction; ovulation from ovarian follicle; positive regulation of angiogenesis; positive regulation of apoptosis; positive regulation of blood vessel diameter; positive regulation of guanylate cyclase activity; regulation of blood vessel size; regulation of sodium ion transport; regulation of systemic arterial blood pressure by endothelin; regulation of the force of heart contraction by chemical signal; removal of superoxide radicals; response to activity; response to amino acid; response to axon injury; response to candesartan; response to corticosterone stimulus; response to cytokine; response to drug; response to estradiol; response to ethanol; response to fructose stimulus; response to high density lipoprotein stimulus; response to hyperoxia; response to hypoxia; response to ketone; response to lead ion; response to lipopolysaccharide; response to mechanical stimulus; response to metal ion; response to organic cyclic compound; response to peptide; response to peptide hormone; response to salt; retina development in camera-type eye; signal transduction; smooth muscle hyperplasia
Research Articles on NOS3
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Product Notes
The NOS3 nos3 (Catalog #AAA2003215) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Nitric Oxide Synthase 3, Endothelial (NOS3) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Nitric Oxide Synthase 3, Endothelial (NOS3) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the NOS3 nos3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSG SEF- AT ILYGSETGRA QSYAQQLGRL FRKAFDPRVL CMDEYDVVSL EHEALVLVVT STFGNGDPPE NGESFAAALM EMSGPYNSSP RPEQHKSYKI RFNSVSCSDP LVSSWRRKRK ESSNTDSAGA LGTLRFCVFG LGSRAYPHFC AFARAVDTRL EELGGERLLQ LGQGDELCGQ EEAFRGWAQA AF. It is sometimes possible for the material contained within the vial of "Nitric Oxide Synthase 3, Endothelial (NOS3), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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