Nerve Growth Factor (NGF) Recombinant Protein | NGF recombinant protein

Recombinant Nerve Growth Factor (NGF)

Cat. Num. AAA2097564

• Gene Names: Ngf; Ngfb; beta-NGF
• Applications: SDS-Page, Western Blot
• Purity: > 90%
• Synonyms: Nerve Growth Factor (NGF); Recombinant Nerve Growth Factor (NGF); NGFB; Beta-NGF; HSAN5; NGF-B; Beta-Nerve Growth Factor; NGF recombinant protein

• Host: E.coli
• Purity/Purification: > 90%
• Form/Format: 100mM NaHCO3, 500mM NaCl, pH8.3, containing 0.01% SKL, 5% Trehalose.
• Concentration: Original Concentration: 200ug/mL (varies by lot)

• Applicable Applications for NGF recombinant protein: Positive Control; Immunogen; SDS-PAGE; Western Blot (WB).
• Organism Species: Rattus norvegicus (Rat)
• Source: Prokaryotic expression
• Residues: Glu19~Gly241
• Tags: N-terminal His Tag
• Subcellular Location: Secreted
• Traits: Freeze-dried powder
• Predicted isoelectric point: 9.4
• Phenomenon explanation: The possible reasons that the actual band size differs from the predicted are as follows:; 1. Splice variants: Alternative splicing may create different sized proteins from the same gene.; 2. Relative charge: The composition of amino acids may affects the charge of the protein.; 3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.; 4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form.; 5. Polymerization of the target protein: Dimerization, multimerization etc.
• Usage: Reconstitute in ddH2O to a concentration of 0.1-1.0 mg/mL. Do not vortex.
• Preparation and Storage: Storage:; Avoid repeated freeze/thaw cycles.; Store at 2-8 degree C for one month. ; Aliquot and store at -80 degree C for 12 months.; ; Stability Test:; The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Sequence

Gene Sequencing (Extract)

(Figure. Gene Sequenceing (Extract))

SDS-Page

(Figure. SDS-PAGE)

Product Categories/Family for NGF recombinant protein

Cytokine; Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 456367246
• NCBI GeneID: 310738
• NCBI Accession #: NP_001263984.1
• NCBI GenBank Nucleotide #: NM_001277055.1
• UniProt Accession #: P25427
• NCBI Official Full Name: beta-nerve growth factor
• NCBI Official Synonym Full Names: nerve growth factor
• NCBI Official Symbol: Ngf
• NCBI Official Synonym Symbols: Ngfb; beta-NGF
• NCBI Protein Information: beta-nerve growth factor
• UniProt Protein Name: Beta-nerve growth factor
• Protein Family: Nerve growth factor
• UniProt Gene Name: Ngf
• UniProt Synonym Gene Names: Ngfb; Beta-NGF

Uniprot Description

Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades through those receptor tyrosine kinase to regulate neuronal proliferation, differentiation and survival. Inhibits metalloproteinase dependent proteolysis of platelet glycoprotein VI.