Forkhead Box Protein O1 (FOXO1) Recombinant Protein | FOXO1 recombinant protein

Recombinant Forkhead Box Protein O1 (FOXO1)

Cat. Num. AAA2018622

• Gene Names: FOXO1; FKH1; FKHR; FOXO1A
• Reactivity: Homo sapiens (Human)
• Applications: SDS-Page, Western Blot
• Purity: > 90%
• Synonyms: Forkhead Box Protein O1 (FOXO1); Recombinant Forkhead Box Protein O1 (FOXO1); FOXO1 recombinant protein

• Host: E.coli
• Reactivity: Homo sapiens (Human)
• Purity/Purification: > 90%
• Form/Format: PBS, pH7.6, containing 0.01% SKL, 5% Trehalose.
• Concentration: Original Concentration: 200ug/mL (varies by lot)
• Sequence: TEQDDLGE GDVHSMVYPP SAAKMASTLP SLSEISNPEN MENLLDNLNL LSSPTSLTVS TQSSPGTMMQ QTPCYSFAPP NTSLNSPSPN YQKYTYGQSS MSPLPQMPIQ TLQDNKSSYG GMSQYNCAPG LLKELLTSDS PPHNDIMTPV DPGVAQPNSR VLGQNVMMGP NSVMSTYGSQ ASHNKMMNPS SHTHPGHAQQ TSAVNGRPLP HTVSTMPHTS GMNRLTQ

• Applicable Applications for FOXO1 recombinant protein: Positive Control; Immunogen; SDS-PAGE; Western Blot (WB).
• Source: Prokaryotic expression
• Residues: Thr333~Gln557
• Tags: N-terminal His Tag
• Subcellular Location: Nucleus, Cytoplasm
• Traits: Freeze-dried powder
• Predicted isoelectric point: 6.4
• Phenomenon explanation: The possible reasons that the actual band size differs from the predicted are as follows:; 1. Splice variants: Alternative splicing may create different sized proteins from the same gene.; 2. Relative charge: The composition of amino acids may affects the charge of the protein.; 3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.; 4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form.; 5. Polymerization of the target protein: Dimerization, multimerization etc.
• Usage: Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
• Preparation and Storage: Storage:; Avoid repeated freeze/thaw cycles.; Store at 2-8°C for one month. ; Aliquot and store at -80°C for 12 months.; ; Stability Test:; The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Gene Sequencing (extract)

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 9257222
• NCBI GeneID: 2308
• NCBI Accession #: NP_002006.2
• NCBI GenBank Nucleotide #: NM_002015.3
• UniProt Accession #: Q12778; O43523; Q5VYC7; Q6NSK6
• Molecular Weight: Predicted Molecular Mass: 27.8kDa; Accurate Molecular Mass: 31kDa as determined by SDS-PAGE reducing conditions.
• NCBI Official Full Name: forkhead box protein O1
• NCBI Official Synonym Full Names: forkhead box O1
• NCBI Official Symbol: FOXO1
• NCBI Official Synonym Symbols: FKH1; FKHR; FOXO1A
• NCBI Protein Information: forkhead box protein O1; forkhead box protein O1A; forkhead, Drosophila, homolog of, in rhabdomyosarcoma
• UniProt Protein Name: Forkhead box protein O1
• Protein Family: Forkhead box protein
• UniProt Gene Name: FOXO1
• UniProt Synonym Gene Names: FKHR; FOXO1A
• UniProt Entry Name: FOXO1_HUMAN

NCBI Description

This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in myogenic growth and differentiation. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma. [provided by RefSeq, Jul 2008]

Uniprot Description

FOXO1A: a transcription factor of the forkhead family. A central regulator of metabolism in several cell types. May play an important role in myogenic growth and differentiation, and in the regulation of lipolysis in adipocytes by controlling the expression of adipose triglyceride lipase (ATGL), the rate-limiting lipolytic enzyme. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma. Contains 1 fork-head domain.

Protein type: Oncoprotein; DNA-binding; Nuclear receptor co-regulator; Transcription factor

Chromosomal Location of Human Ortholog: 13q14.1

Cellular Component: nucleoplasm; mitochondrion; cytoplasm; nucleus; cytosol

Molecular Function: protein binding; protein phosphatase 2A binding; sequence-specific DNA binding; ubiquitin protein ligase binding; chromatin binding

Biological Process: fat cell differentiation; transcription from RNA polymerase II promoter; nerve growth factor receptor signaling pathway; apoptosis; positive regulation of apoptosis; positive regulation of transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter; thermoregulation; positive regulation of gluconeogenesis; positive regulation of autophagy; epidermal growth factor receptor signaling pathway; blood vessel development; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; negative regulation of stress-activated MAPK cascade; cellular response to starvation; negative regulation of fat cell differentiation; endocrine pancreas development; protein amino acid acetylation; cellular response to insulin stimulus; positive regulation of protein catabolic process; insulin receptor signaling pathway; innate immune response; autophagy; cell glucose homeostasis; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; response to DNA damage stimulus; negative regulation of apoptosis

Disease: Rhabdomyosarcoma 2