Mouse nuclear factor (erythroid-derived 2) -like 2 ELISA Kit | NFE2L2 elisa kit

Mouse Nuclear factor erythroid 2-related factor 2, Nrf2 ELISA Kit

Cat. Num. AAA705641

• Gene Names: Nfe2l2; Nrf2; AI194320
• Reactivity: Mouse
• Synonyms: nuclear factor (erythroid-derived 2) -like 2; Mouse Nuclear factor erythroid 2-related factor 2; Nrf2 ELISA Kit; Mouse Nuclear factor erythroid 2-related factor 2 (Nrf2) ELISA Kit; NRF2; NF-E2-related factor 2; nuclear factor erythroid 2-like 2; NFE2L2 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mouse NFE2L2. No significant cross-reactivity or interference between Mouse NFE2L2 and analogues was observed.
• Sequence Length: 597

• Samples: Serum, plasma and cell culture supernates
• Detection Range: 0.312 ng/ml-20 ng/ml
• Sensitivity: 0.078 ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Protein Biological Process 1: Transcription/Transcription regulation
• Protein Biological Process 3: Transcription
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NFE2L2 elisa kit

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nuclear factor erythroid 2-related factor 2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Nuclear factor erythroid 2-related factor 2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain Nuclear factor erythroid 2-related factor 2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Nuclear factor erythroid 2-related factor 2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 6754832
• NCBI GeneID: 18024
• NCBI Accession #: NP_035032.1
• NCBI GenBank Nucleotide #: NM_010902.3
• UniProt Accession #: Q60795; Q3UQK0
• Molecular Weight: 66,901 Da
• NCBI Official Full Name: nuclear factor erythroid 2-related factor 2
• NCBI Official Synonym Full Names: nuclear factor, erythroid derived 2, like 2
• NCBI Official Symbol: Nfe2l2
• NCBI Official Synonym Symbols: Nrf2; AI194320
• NCBI Protein Information: nuclear factor erythroid 2-related factor 2; NFE2-related factor 2; NF-E2-related factor 2
• UniProt Protein Name: Nuclear factor erythroid 2-related factor 2
• Protein Family: Nuclear factor erythroid 2-related factor
• UniProt Gene Name: Nfe2l2
• UniProt Synonym Gene Names: Nrf-2; Nrf2; NF-E2-related factor 2; NFE2-related factor 2
• UniProt Entry Name: NF2L2_MOUSE

NCBI Description

This gene encodes a transcription factor which is a member of a small family of basic leucine zipper (bZIP) proteins. The encoded transcription factor regulates genes which contain antioxidant response elements (ARE) in their promoters; many of these genes encode proteins involved in response to injury and inflammation which includes the production of free radicals. [provided by RefSeq, Sep 2015]

Uniprot Description

Function: Transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. Important for the coordinated up-regulation of genes in response to oxidative stress. May be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region.1 PublicationManual assertion based on experiment in:Ref.6