Human colony stimulating factor 3 (granulocyte) ELISA Kit | CSF3 elisa kit

Human Granulocyte Colony Stimulating Factor, G-CSF ELISA Kit

Cat. Num. AAA701149

• Gene Names: CSF3; GCSF; CSF3OS; C17orf33
• Reactivity: Human
• Synonyms: colony stimulating factor 3 (granulocyte); Human Granulocyte Colony Stimulating Factor; G-CSF ELISA Kit; G-CSF; GCSF; MGC45931; colony stimulating factor 3; filgrastim; granulocyte colony stimulating factor; lenograstim; pluripoietin; CSF3 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human CSF3. No significant cross-reactivity or interference between Human CSF3 and analogues was observed.
• Sequence Length: 207

• Samples: Serum, plasma, cell culture supernates, tissue homogenates
• Detection Range: 0.312 ng/ml-20 ng/ml
• Sensitivity: 0.078 ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CSF3 elisa kit

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to G-CSF. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for G-CSF and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain G-CSF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of G-CSF in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4503079
• NCBI GeneID: 1440
• NCBI Accession #: NP_000750.1
• NCBI GenBank Nucleotide #: NM_000759.3
• UniProt Accession #: P09919; A8MXR7
• Molecular Weight: 22,293 Da
• NCBI Official Full Name: granulocyte colony-stimulating factor isoform a
• NCBI Official Synonym Full Names: colony stimulating factor 3 (granulocyte)
• NCBI Official Symbol: CSF3
• NCBI Official Synonym Symbols: GCSF; CSF3OS; C17orf33
• NCBI Protein Information: granulocyte colony-stimulating factor; filgrastim; lenograstim; pluripoietin
• UniProt Protein Name: Granulocyte colony-stimulating factor
• Protein Family: Granulocyte colony-stimulating factor
• UniProt Gene Name: CSF3
• UniProt Synonym Gene Names: C17orf33; GCSF; G-CSF
• UniProt Entry Name: CSF3_HUMAN

NCBI Description

The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes. The active protein is found extracellularly. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, May 2010]

Uniprot Description

Function: Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.