Human insulin-like growth factor 2 mRNA binding protein 2 ELISA Kit | IGF2BP2 elisa kit

Human Insulin-like growth factor 2 mRNA-binding protein 2, IGF2BP2 ELISA Kit

Cat. Num. AAA922044

• Gene Names: IGF2BP2; IMP2; IMP-2; VICKZ2
• Reactivity: Human
• Synonyms: insulin-like growth factor 2 mRNA binding protein 2; Human Insulin-like growth factor 2 mRNA-binding protein 2; IGF2BP2 ELISA Kit; Human Insulin-like growth factor 2 mRNA-binding protein 2 (IGF2BP2) ELISA kit; IMP-2; IMP2; VICKZ2; p62; IGF-II mRNA-binding protein 2; IGF2BP2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human IGF2BP2. No significant cross-reactivity or interference between Human IGF2BP2 and analogues was observed.
• Sequence Length: 556

• Samples: Serum, plasma, tissue homogenates, Cell lysates
• Detection Range: 125 pg/ml-8000 pg/ml
• Sensitivity: 31.25 pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Protein Biological Process 1: Transcription/Transcription regulation
• Protein Biological Process 3: Translation regulation
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IGF2BP2 elisa kit

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to IGF2BP2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for IGF2BP2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain IGF2BP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of IGF2BP2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 56118219
• NCBI GeneID: 10644
• NCBI Accession #: NP_001007226.1
• NCBI GenBank Nucleotide #: NM_001007225.1
• UniProt Accession #: Q9Y6M1; A0A4Z0; B3FTN2; B3FTN3; B3FTN4
• Molecular Weight: 66,121 Da
• NCBI Official Full Name: insulin-like growth factor 2 mRNA-binding protein 2 isoform b
• NCBI Official Synonym Full Names: insulin-like growth factor 2 mRNA binding protein 2
• NCBI Official Symbol: IGF2BP2
• NCBI Official Synonym Symbols: IMP2; IMP-2; VICKZ2
• NCBI Protein Information: insulin-like growth factor 2 mRNA-binding protein 2; VICKZ family member 2; IGF2 mRNA-binding protein 2; IGF-II mRNA-binding protein 2
• UniProt Protein Name: Insulin-like growth factor 2 mRNA-binding protein 2
• Protein Family: Insulin-like growth factor 2 mRNA-binding protein
• UniProt Gene Name: IGF2BP2
• UniProt Synonym Gene Names: IMP2; VICKZ2; IGF2 mRNA-binding protein 2; IMP-2
• UniProt Entry Name: IF2B2_HUMAN

NCBI Description

This gene encodes a member of the IGF-II mRNA-binding protein (IMP) family. The protein encoded by this gene contains four KH domains and two RRM domains. It functions by binding to the 5' UTR of the insulin-like growth factor 2 (IGF2) mRNA and regulating IGF2 translation. Alternative promoter usage and alternate splicing result in multiple variants encoding different isoforms. [provided by RefSeq, May 2014]

Uniprot Description

IGF2BP2: Binds to the 5'-UTR of the insulin-like growth factor 2 (IGF2) mRNAs. Binding is isoform-specific. May regulate translation of target mRNAs. Belongs to the RRM IMP/VICKZ family. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Translation; RNA-binding

Chromosomal Location of Human Ortholog: 3q27.2

Cellular Component: cytoskeleton; cytoplasm; cytosol; nucleus

Molecular Function: protein binding; mRNA 3'-UTR binding; nucleotide binding; translation regulator activity; mRNA 5'-UTR binding

Biological Process: anatomical structure morphogenesis; mRNA transport; negative regulation of translation; gene expression; regulation of cytokine biosynthetic process

Disease: Diabetes Mellitus, Noninsulin-dependent