Rat Nuclear Factor of Activated T-Cells, Cytoplasmic 2 ELISA Kit | NFATC2 elisa kit

Rat Nuclear Factor of Activated T-Cells, Cytoplasmic 2 ELISA Kit

Cat. Num. AAA59129

• Gene Names: NFATC2; NFAT1; NFATP
• Reactivity: Rat
• Synonyms: Nuclear Factor of Activated T-Cells; Cytoplasmic 2; Rat Nuclear Factor of Activated T-Cells; Cytoplasmic 2 ELISA Kit; NFATC2 elisa kit

• Reactivity: Rat
• Specificity: No significant cross-reactivity or interference between Rat NFATC2 and analogues was observed.
• Sequence Length: 925

• Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine
• Assay Type: Sandwich
• Detection Range: 31.2pg/ml-1000pg/ml.
• Sensitivity: 5.0pg/ml.
• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated NFATC2 concentrations in Rat serum, plasma and other body fluids. Using Purified Rat NFATC2 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add NFATC2 and NFATC2 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of NFATC2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for NFATC2 elisa kit

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated NFATC2 concentrations in Rat serum, plasma and other body fluids. Using Purified Rat NFATC2 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add NFATC2 and NFATC2 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of NFATC2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 27886541
• NCBI GeneID: 4773
• NCBI Accession #: NP_775114.1
• NCBI GenBank Nucleotide #: NM_173091.3
• UniProt Accession #: Q13469; Q13468; Q5TFW7; Q5TFW8; Q9NPX6; Q9NQH3; Q9UJR2; B5B2N8; B5B2N9; B5B2P2; B5B2P3
• Molecular Weight: 100,146 Da
• NCBI Official Full Name: nuclear factor of activated T-cells, cytoplasmic 2 isoform C
• NCBI Official Synonym Full Names: nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2
• NCBI Official Symbol: NFATC2
• NCBI Official Synonym Symbols: NFAT1; NFATP
• NCBI Protein Information: nuclear factor of activated T-cells, cytoplasmic 2; NF-ATc2; NFAT pre-existing subunit; T cell transcription factor NFAT1; T-cell transcription factor NFAT1; NFAT transcription complex, preexisting component; preexisting nuclear factor of activated T-cells 2; nuclear factor of activated T-cells, preexisting component
• UniProt Protein Name: Nuclear factor of activated T-cells, cytoplasmic 2
• Protein Family: Nuclear factor of activated T-cells
• UniProt Gene Name: NFATC2
• UniProt Synonym Gene Names: NFAT1; NFATP; NF-ATc2; NFATc2; NF-ATp
• UniProt Entry Name: NFAC2_HUMAN

NCBI Description

This gene is a member of the nuclear factor of activated T cells (NFAT) family. The product of this gene is a DNA-binding protein with a REL-homology region (RHR) and an NFAT-homology region (NHR). This protein is present in the cytosol and only translocates to the nucleus upon T cell receptor (TCR) stimulation, where it becomes a member of the nuclear factors of activated T cells transcription complex. This complex plays a central role in inducing gene transcription during the immune response. Alternate transcriptional splice variants encoding different isoforms have been characterized. [provided by RefSeq, Apr 2012]

Uniprot Description

NFAT1: a transcription factor that plays a role in the inducible expression of cytokine genes in T cells, especially in the induction of the IL-2, IL-3, IL-4, TNF-alpha and GM-CSF. It is present in a phosphorylated the cytosol of unstimulated T cells. After T-cell activation, it is dephosphorylated by calcineurin and translocates to the nucleus as a part of a transcription complex. Two splice variant isoforms have been identified.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 20q13.2

Cellular Component: nucleoplasm; cytoplasm; plasma membrane; ribonucleoprotein complex; cytosol; nucleus; actin cytoskeleton

Molecular Function: protein binding; DNA binding; transcription factor activity

Biological Process: response to drug; B cell receptor signaling pathway; cell migration; regulation of transcription, DNA-dependent; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; positive regulation of B cell proliferation; cytokine production; innate immune response; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; response to DNA damage stimulus