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Typical Testing Data/Standard Curve (for reference only) (Fig.1. Human FSH Standard Curve.)

Human FSH ELISA Kit | FSH elisa kit

Human FSH ELISA Kit

Gene Names
FSHB; HH24
Reactivity
Human
Synonyms
FSH; Human FSH ELISA Kit; Follicle stimulating hormone alpha chain; Follicle Stimulating Hormone Beta; Follicle stimulating hormone beta polypeptide; Follicle stimulating hormone beta subunit; Follitropin alpha chain; Follitropin beta chain; Follitropin subunit beta; FSH A; FSH alpha; FSH B; FSH beta; FSHA; FSHB; FSH elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Specificity
Human FSH ELISA Kit has high sensitivity and excellent specificity for detection of Human FSH. No significant cross-reactivity or interference between Human FSH and analogues was observed.
Sequence Length
129
Assay Type
Sandwich ELISA (Quantitative)
Samples
Serum, Plasma, Other Biological Fluids
Detection Range
2 IU/L-70 IU/L
Detection Method
Colorimetric
Limit of Detection
1.0 IU/L
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for
Preparation and Storage
Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Human FSH Standard Curve.)

Typical Testing Data/Standard Curve (for reference only) (Fig.1. Human FSH Standard Curve.)
Related Product Information for FSH elisa kit
Description: Human FSH ELISA Kit employs a two-site sandwich ELISA to quantitate FSH in samples. An antibody specific for FSH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FSH present is bound by the immobilized antibody. After removing any unbound substances, a HRP conjugated antibody specific for FSH is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FSH bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: Follicle Stimulating Hormone (FSH) is a glygoprotein produced by the anterior pituitary gland. In the female, FSH stimulates follicular growth, prepares ovarian follicles for action by LH and enhances the LH induced release of estrogen. FSH levels are elevated after menopause, castration and in premature ovarian failure. Although there are significant exceptions ovarian failure is indicated when random FSH concentrations exceed 40 mIU/ml. In the male, FSH stimulates seminiferous tubule and testicular growth and is involved in the early stages of spermatogenesis. Oligospermic males usually have elevated FSH levels. Tumors of the testes generally depress serum FSH concentrations, but levels of LH are elevated. High levels of FSH in men may be found in primary testicular failure and Klinefelter syndrome. Elevated concentrations are also present in cases of starvation, renal failure, hyperthyroidism, and cirrhosis.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
follicle-stimulating hormone
NCBI Official Synonym Full Names
follicle stimulating hormone subunit beta
NCBI Official Symbol
FSHB
NCBI Official Synonym Symbols
HH24
NCBI Protein Information
follitropin subunit beta
UniProt Protein Name
Follitropin subunit beta
UniProt Gene Name
FSHB
UniProt Synonym Gene Names
FSH-B; FSH-beta
UniProt Entry Name
FSHB_HUMAN

NCBI Description

The pituitary glycoprotein hormone family includes follicle-stimulating hormone, luteinizing hormone, chorionic gonadotropin, and thyroid-stimulating hormone. All of these glycoproteins consist of an identical alpha subunit and a hormone-specific beta subunit. This gene encodes the beta subunit of follicle-stimulating hormone. In conjunction with luteinizing hormone, follicle-stimulating hormone induces egg and sperm production. Alternative splicing results in two transcript variants encoding the same protein. [provided by RefSeq, Jul 2008]

Uniprot Description

FSHB: Stimulates development of follicle and spermatogenesis in the reproductive organs. Defects in FSHB are a cause of isolated follicle- stimulating hormone deficiency (IFSHD). Selective follicle-stimulating hormone deficiency is an uncommon cause of infertility, producing amenorrhea and hypogonadism in women and oligo or azoospermia with normal testosterone levels in normally virilised men. Belongs to the glycoprotein hormones subunit beta family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 11p13

Cellular Component: cytoplasm; extracellular region

Molecular Function: follicle-stimulating hormone activity; protein binding; hormone activity

Biological Process: progesterone biosynthetic process; follicle-stimulating hormone signaling pathway; peptide hormone processing; female gamete generation; Sertoli cell proliferation; female pregnancy; signal transduction; regulation of osteoclast differentiation; cellular protein metabolic process; transforming growth factor beta receptor signaling pathway; positive regulation of cell proliferation; positive regulation of bone resorption; positive regulation of transcription from RNA polymerase II promoter; positive regulation of cell migration

Disease: Follicle-stimulating Hormone Deficiency, Isolated

Research Articles on FSH

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Product Notes

The Human FSH fshb (Catalog #AAA9711571) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9711571 ELISA Kit recognizes Human FSH. It is sometimes possible for the material contained within the vial of "FSH, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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