Human Nuclear Factor Of Activated T-Cells, Cytoplasmic 2 ELISA Kit | NFATC2 elisa kit

Human NFATC2 (Nuclear Factor Of Activated T-Cells, Cytoplasmic 2) ELISA Kit

Cat. Num. AAA2533415

• Gene Names: NFATC2; NFAT1; NFATP
• Reactivity: Human
• Synonyms: Nuclear Factor Of Activated T-Cells; Cytoplasmic 2; Human NFATC2 (Nuclear Factor Of Activated T-Cells; Cytoplasmic 2) ELISA Kit; NFATC2 elisa kit

• Reactivity: Human
• Specificity: This kit recognizes natural and recombinantHumanNFATC2. No significant cross-reactivity or interference between HumanNFATC2 and analogues was observed.
• Sequence Length: 925

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 31.25-2000pg/mL
• Sensitivity: Min: 18.75pg/mL; Max: 2000pg/mL
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NFATC2 elisa kit

Intended Uses: This ELISA kit applies to the invitro quantitative determination of HumanNFATC2concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to NFATC2. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for NFATC2and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain NFATC2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of NFATC2.You can calculate the concentration of NFATC2in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 27886541
• NCBI GeneID: 4773
• NCBI Accession #: NP_775114.1
• NCBI GenBank Nucleotide #: NM_173091.3
• Molecular Weight: 76,700 Da
• NCBI Official Full Name: nuclear factor of activated T-cells, cytoplasmic 2 isoform C
• NCBI Official Synonym Full Names: nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2
• NCBI Official Symbol: NFATC2
• NCBI Official Synonym Symbols: NFAT1; NFATP
• NCBI Protein Information: nuclear factor of activated T-cells, cytoplasmic 2
• UniProt Protein Name: Nuclear factor of activated T-cells, cytoplasmic 2
• Protein Family: Nuclear factor of activated T-cells
• UniProt Gene Name: NFATC2
• UniProt Synonym Gene Names: NFAT1; NFATP; NF-ATc2; NFATc2; NF-ATp
• UniProt Entry Name: NFAC2_HUMAN

NCBI Description

This gene is a member of the nuclear factor of activated T cells (NFAT) family. The product of this gene is a DNA-binding protein with a REL-homology region (RHR) and an NFAT-homology region (NHR). This protein is present in the cytosol and only translocates to the nucleus upon T cell receptor (TCR) stimulation, where it becomes a member of the nuclear factors of activated T cells transcription complex. This complex plays a central role in inducing gene transcription during the immune response. Alternate transcriptional splice variants encoding different isoforms have been characterized. [provided by RefSeq, Apr 2012]

Uniprot Description

NFAT1: a transcription factor that plays a role in the inducible expression of cytokine genes in T cells, especially in the induction of the IL-2, IL-3, IL-4, TNF-alpha and GM-CSF. It is present in a phosphorylated the cytosol of unstimulated T cells. After T-cell activation, it is dephosphorylated by calcineurin and translocates to the nucleus as a part of a transcription complex. Two splice variant isoforms have been identified.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 20q13.2

Cellular Component: actin cytoskeleton; cytoplasm; cytosol; nucleoplasm; nucleus; plasma membrane; ribonucleoprotein complex

Molecular Function: chromatin binding; DNA binding; protein binding; transcription factor activity; transcription factor binding

Biological Process: B cell receptor signaling pathway; calcineurin-NFAT signaling pathway; cell migration; cytokine production; innate immune response; myotube cell development; negative regulation of transcription from RNA polymerase II promoter; positive regulation of B cell proliferation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of transcription, DNA-dependent; response to DNA damage stimulus; response to drug; stimulatory C-type lectin receptor signaling pathway; transcription from RNA polymerase II promoter