Rabbit Noradrenaline ELISA Kit | NA elisa kit

Rabbit Noradrenaline (NA) ELISA Kit

Cat. Num. AAA284153

• Gene Names: SLC6A2; NET; NAT1; NET1; SLC6A5
• Reactivity: Rabbit
• Synonyms: Noradrenaline; Rabbit Noradrenaline (NA) ELISA Kit; NA elisa kit

• Reactivity: Rabbit
• Specificity: This assay has high sensitivity and excellent specificity for detection of Rabbit NA. No significant cross-reactivity or interference between Rabbit NA and analogues was observed.
• Sequence Length: 617

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for NA elisa kit

Noradrenaline is the neurotransmitter of the peripheral sympathetic nervous system, responsible for so-called adrenergic neurotransmission, at nerve endings in the heart, in smooth (involuntary) muscle, and in many glands. It is one of the catecholamines. Tyrosine, an amino acid present in body fluids, is taken up into the adrenergic nerve terminal where it is acted upon by an enzyme, tyrosine hydroxylase, to form DOPA; this is converted to dopamine and in turn to noradrenaline. The neurotransmitter is stored in small vesicles, awaiting release. Arrival of the impulse at the nerve terminal releases vesicles, freeing the noradrenaline to exert its effects. To terminate the action of the transmitter, some noradrenaline is oxidized to inactive material, but most is taken back up into the nerve terminal and stored for later use. There are two types of cell membrane receptors with which noradrenaline interacts, termed a and b, each group having several subtypes.

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate NA in samples. An antibody specific for NA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NA bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 189258
• NCBI GeneID: 6530
• Molecular Weight: 57,370 Da
• NCBI Official Full Name: noradrenaline transporter
• NCBI Official Synonym Full Names: solute carrier family 6 (neurotransmitter transporter), member 2
• NCBI Official Symbol: SLC6A2
• NCBI Official Synonym Symbols: NET; NAT1; NET1; SLC6A5
• NCBI Protein Information: sodium-dependent noradrenaline transporter
• UniProt Protein Name: Sodium-dependent noradrenaline transporter
• Protein Family: Neuraminidase
• UniProt Gene Name: SLC6A2
• UniProt Synonym Gene Names: NAT1; NET1; SLC6A5; NET
• UniProt Entry Name: SC6A2_HUMAN

NCBI Description

This gene encodes a member of the sodium:neurotransmitter symporter family. This member is a multi-pass membrane protein, which is responsible for reuptake of norepinephrine into presynaptic nerve terminals and is a regulator of norepinephrine homeostasis. Mutations in this gene cause orthostatic intolerance, a syndrome characterized by lightheadedness, fatigue, altered mentation and syncope. Alternatively spliced transcript variants encoding different isoforms have been identified in this gene.[provided by RefSeq, Feb 2010]

Uniprot Description

SLC6A2: Amine transporter. Terminates the action of noradrenaline by its high affinity sodium-dependent reuptake into presynaptic terminals. Defects in SLC6A2 are a cause of orthostatic intolerance (OI). OI is a syndrome characterized by lightheadedness, fatigue, altered mentation and syncope. It is associated with postural tachycardia. Plasma norepinephrine concentration is abnormally high. Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A2 subfamily.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; Transporter; Transporter, SLC family

Chromosomal Location of Human Ortholog: 16q12.2

Cellular Component: integral to plasma membrane; membrane; neuron projection; plasma membrane

Molecular Function: dopamine:sodium symporter activity; monoamine transmembrane transporter activity; norephinephrine:sodium symporter activity; protein binding

Biological Process: dopamine uptake; monoamine transport; norepinephrine transport; response to pain; synaptic transmission; transmembrane transport; transport

Disease: Orthostatic Intolerance