Mouse Sodium-dependent noradrenaline transporter ELISA Kit | Slc6a2 elisa kit

Mouse Sodium-dependent noradrenaline transporter ELISA Kit

Cat. Num. AAA765810

• Gene Names: SLC6A2; NET; NAT1; NET1; SLC6A5
• Reactivity: Mouse
• Synonyms: Sodium-dependent noradrenaline transporter; Mouse Sodium-dependent noradrenaline transporter ELISA Kit; Slc6a2/Sodium-dependent noradrenaline transporter/Norepinephrine transporter/NET/Solute carrier family 6 member 2; Slc6a2 elisa kit

• Reactivity: Mouse
• Sequence Length: 628

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for Slc6a2 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Mouse Slc6a2 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse Slc6a2 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse Slc6a2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse Slc6a2 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 289191377
• NCBI GeneID: 6530
• NCBI Accession #: NP_001165975.1
• NCBI GenBank Nucleotide #: NM_001172504.1
• Molecular Weight: 57,370 Da
• NCBI Official Full Name: sodium-dependent noradrenaline transporter isoform 1
• NCBI Official Synonym Full Names: solute carrier family 6 (neurotransmitter transporter), member 2
• NCBI Official Symbol: SLC6A2
• NCBI Official Synonym Symbols: NET; NAT1; NET1; SLC6A5
• NCBI Protein Information: sodium-dependent noradrenaline transporter
• UniProt Protein Name: Sodium-dependent noradrenaline transporter
• Protein Family: Sodium-dependent noradrenaline transporter
• UniProt Gene Name: SLC6A2
• UniProt Synonym Gene Names: NAT1; NET1; SLC6A5; NET
• UniProt Entry Name: SC6A2_HUMAN

NCBI Description

This gene encodes a member of the sodium:neurotransmitter symporter family. This member is a multi-pass membrane protein, which is responsible for reuptake of norepinephrine into presynaptic nerve terminals and is a regulator of norepinephrine homeostasis. Mutations in this gene cause orthostatic intolerance, a syndrome characterized by lightheadedness, fatigue, altered mentation and syncope. Alternatively spliced transcript variants encoding different isoforms have been identified in this gene.[provided by RefSeq, Feb 2010]

Uniprot Description

SLC6A2: Amine transporter. Terminates the action of noradrenaline by its high affinity sodium-dependent reuptake into presynaptic terminals. Defects in SLC6A2 are a cause of orthostatic intolerance (OI). OI is a syndrome characterized by lightheadedness, fatigue, altered mentation and syncope. It is associated with postural tachycardia. Plasma norepinephrine concentration is abnormally high. Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A2 subfamily.

Protein type: Transporter; Transporter, SLC family; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 16q12.2

Cellular Component: integral to plasma membrane; membrane; neuron projection; plasma membrane

Molecular Function: dopamine:sodium symporter activity; monoamine transmembrane transporter activity; norephinephrine:sodium symporter activity; protein binding

Biological Process: dopamine uptake; monoamine transport; norepinephrine transport; response to pain; synaptic transmission; transmembrane transport; transport

Disease: Orthostatic Intolerance